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unicellular archae unicellular bacteria |
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Eukarya (unicellular)Microbes: |
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Algae, Fungi (*yeast), Protozoans (protists) |
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Eukarya (multicellular)Microbes: |
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Algae, Helminths (roundworms, tapeworms) |
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What reveals the 3 microbial domains? |
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small subunit rRNA sequences reveal three deeply branching domains of life |
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Nucleic acid+ protein coat (=capsid) req. host to multiply |
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Enveloped= host cell derived outer membrane non= naked |
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What is viroids? specific primarily for: req: |
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Nucleic acid only No capsid Plant disease req host rna dependent rna polymerase for replication |
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Size: Prochlorococcus marinus Staphylococcus aureus Escherichia coli Bacillus megaterium Red blood cell |
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.4 micro m .9 micro m 2.5 4 8 |
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Light microscopy can see as small as bright field- |
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Electron microscopy can be |
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10 & -8 scanning, transmission |
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10-9 3D live samples lever goes across sample, no vacuum, can see molecules (benzene) viruses |
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Xray crystallography/ NMR spectroscopy |
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10 -10m= angstroms x-ray= must be crystallized NMR- must be in solution, takes a long time= not normal conditions |
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slicing, view thru image not just outside fires electrons= no live samples |
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