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• Proteins that allow chemical reactions to take place • Acts on a substrate that fits specifically into the enzyme’s active site • Can break substrates into two products or merge two substrates into one • Doesn’t get changed in the process—just a catalyst, speeds up reactions • Lower activation energy of a reaction |
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• Acted on by enzymes by going into the active site |
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• The process of speeding up reactions to a greater rate than they would happen normally • Enzymes do this |
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• Couple energetically unfavorable reactions with energetically favorable ones • Like a waterfall powering a turbine of a generator do work • Powers active transport, can make complex molecules |
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• Molecules that can store a little energy • ATP is the most important one for a cell o Has energy in covalent bonds attached to phosphate groups o When one of these bonds is broken ADP and releases energy • Some carry energy in extra electrons like NAD+ NADH; NADP+ NADPH; FAD FADH2 • Energy is being carried in the second form and is released when the hydrogens are taken away |
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• Process where cells break down food and make it into usable energy • Starts with glucose, produces ATP and CO2 • Glycolysis citric acid cycle (Krebs cycle) ETC oxidative phosphorylation • C6H12O6 + 6 O2 6 H2O + 6CO2 + energy |
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• First step of respiration • glucose + 2 NAD+ + 2 ADP 2 NADH + 2 ATP + 2 pyruvate • Three steps o Cell puts in two ATP and glucose is rearranged into six-carbon sugar o Cleavage: cell breaks six-carbon sugar into two three-carbon intermediate sugars o Those molecules made into two pyruvate—two NAD+ NADH and 4 ATP made • Only part of respiration that prokaryotic cells can do because it doesn’t require mitochondria • Diagram page 84 |
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• Product of glycolysis • Has three carbons • Converted to acetyl CoA before Krebs cycle |
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• Has to happen for the Krebs cycle to work • Three enzymes from mitochondria used, called pyruvate dehydrogenase complex • Acetyl CoA is a two-carbon sugar attached to a coenzyme • Pyruvate + NAD+ + coenzyme A NADH + CO2 + acetyl CoA (twice per glucose) • Acetyl CoA can also come from fat molecules |
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Citric Acid Cycle (Krebs Cycle) |
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• Release energy from pyruvate molecules and release CO2 • The acetyl CoA attached to oxaloacetate citrate name of cycle • Energy taken over 10 steps original oxaloacetate can start over with another acetyl CoA • Acetyl CoA + 2 H2O + 2 NAD+ + FAD + GDP 2 CO2 + 2 NADH + FADH2 + GTP + coenzyme A • Energy gains: 3 NAD+ 3 NADH, 1 FAD 1 FADH2, 1 GDP 1 GTP • End of this, all carbons from original glucose released as CO2 • Diagram page 86 |
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• A nonprotein thing needed to make an enzyme function |
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Oxidative Phosphorylation |
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• Energy stored in electron carriers NADH and FADH2 is converted to usable energy in ATP • Concentration gradient of H+ created by ETC • H+ travel through ATP synthase to fix gradient energy makes ADP into ~30 ATP • Relies on oxygen—that’s why we need oxygen • Diagram page 87 |
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• Series of proteins embedded in inner membrane of mitochondria • Transports electrons from NADH/FADH2 down chain, they end up on an oxygen water • Hydrogen protons H+ are transported to other side of membrane |
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• Process where NAD+ is regenerated from NADH in anaerobic conditions—without oxygen • Alcoholic and lactic acid fermentation are two most common |
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• Pyruvate from glycolysis 2-carbon ethanol, makes two additional ATP and releases 1 CO2 • Regenerates two NAD+ from NADH so they can be used for glycolysis • Yeast and bacteria do this • Anaerobic |
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• Pyruvate from glycolysis lactate, makes two additional ATP • Regernates two NAD+ from NADH so they can be used for glycolysis • Animals do this, lactate buildiup cramp • Anaerobic |
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• Plants capture energy from sunlight, then use it to make a big sugar molecule to store energy • Capture carbon dioxide and release oxygen • 6 H2O + 6 CO2 + light energy C6H12O6 + H2O • Light reactions and dark reactions |
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• Organisms that can produce their own food (like plants with photosynthesis) |
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• Organisms that have to have some sort of outside food source of organic molecules |
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• Parts of photosynthesis that rely on sunlight and use chlorophyll • Light energy from sun usable chemical energy • Happen in thylakoid membranes of chloroplast • Use energy of excited electrons through photosystems |
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• Key molecule of light reactions • Pigment in the chloroplast that gives leaves green color • When hit by light, an electron gets excited and has more energy • Accessory pigments—pass their excited electrons to cholorphyll • Passes excited electrons to first step of ETC, primary electron acceptor |
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• Series of electron transport chains that photosynthesis uses to harness energy from excited electrons • Have proteins and pigments like chlorophyll • Use cytochrome complex to pass electrons • As electrons passed, water broken into oxygen/hydrogen and H+ concentration gradient builds ATP • Other elctron transport chain uses NADP+ • End of transport chains, NADP+ receives the electron NADPH |
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• Energy from light reactions harnessed sugar molecules like glucose • Happen in stroma of chloroplast • Only rely on NADPH and ATP don’t need sunlight “dark” reactions • Biggest part is Calvin Cycle |
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• Primary component of dark reactions • Cyclical • Takes carbon in organic molecule, opposite of Krebs cycle • Oxygen from CO2 combined with hydrogen to make water • Diagram on page 91 • ATP and NADPH NADP+ and glyceraldehyde-3-phosphate molecules o Some help make glucose and such, some go back to the cycle to make more ribulose • 3 CO2 + 9 ATP + 6 NADPH glyceralhedyde-3-phosphate + 12 H2O |
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• Protein that converts gaseous carbon into solid carbon • Crucial part of Calvin cycle • CO2 5-carbon ribulose biphosphate 6-carbon intermediate sugar two 3-carbon sugars • Has to do this three times for every three-carbon sugar from Calvin cycle • Some plants use PEP carboxylase instead, which is more efficient |
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• Separate times when two reactions of photosynthesis take place • Only open stroma at night so that they can take in CO2 without water evaporating • Light reactions during the day ATP and NADPH; dark reactions at night |
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• Conditions where there is no oxygen present • fermentation |
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