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ANS 327, Advanced Repro
In lecture notes
45
Agriculture
Undergraduate 4
10/21/2008

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Cards

Term
Which species can AI be performed on?
Definition
All livestock, dogs, cats, poultry, lab animals, invertibrates
Term
What did Anton von Leeuwenhoek do, and when?
Definition
1677: Created simple microscope and discovered/described semen in sperm as "animalcules"
Term
Why are the 1300s important to AI?
Definition
Some documentation leading people to believe that the Arabs were AIing horses
Term
What did Lazaro Spallanzani contribute to AI and when?
Definition
1780: First successful AI (using dogs) and early studies of freezing and thawing stallion semen
Term
What is E.I. Ivanoff known for? When?
Definition
Early 1900s: First to AI horses, cattle and sheep
Term
What is G. Amantea known for and when?
Definition
1914: Developed the first artificial vagina (used dogs)
Term
What event happened in 1936?
Definition
First AI cooperative in Denmark
Term
What was developed in 1937?
Definition
Rectovaginal technique in cattle by Danish vets
Term
What did E.J. Perry do? When?
Definition
1938: Formed first AI cooperative in US
Term
What is credited to P.H. Phillips and H.A. Lardy? When?
Definition
Late 1930s: Developed egg-yolk based extender
Term
What is A.M. Sorenson known for?
Definition
1940: Introduced the plastic straw for storing semen
Term
What happened in the 1940s?
Definition
Electroejaculator created
Term
What is Christopher Polge known for?
Definition
1949: Discovered Glycerol as cryoprotectant (still used for semen and embryos)
Term
What is important about 1954?
Definition
Waterloo cattle breeding assoc. was the first 100% frozen semen breeding operation.
Term
Significance of 1957?
Definition
American Breeders Service started using liquid nitrogen to freeze/store semen instead of dry ice
Term
What did R. Cassou do? When?
Definition
1964: Developed Cassou inseminating "gun", refined the plastic straw to make the standard French straw or medium straw (0.5 mL)
Term
What happened in 1972?
Definition
Shift from glass ampules to plastic straws
Term
What are the advantages/ benefits of AI?
Definition
Genetic improvement; control of disease; accurate records; increased $ return;
Term
What are the disadvantages of AI?
Definition
Need special training; special facilities for heat detection/AI; labor intensive; NUMBER OF ANIMALS MAY BE LIMITING;
Term
What is the most common AI technique in cattle?
Definition
Rectovaginal
Term
Where is semen deposited in cattle?
Definition
Uterine body, NOT UTERINE HORN
Term
What is the rule about inseminating cows that are heat checked 2x/day?
Definition
AI cows in am that show heat the previous night, AI cows that show heat in am the following night
Term
Why do you follow the am/pm rule in cattle?
Definition
cow has 21 day estrous w/ short estrus of 14-18 hrs and ovulation occurs 12-16 hours later (during metestrus) and bull sperm must be in tract for 12 hrs to capacitate and become viable
Term
What is capacitation?
Definition
Removal of cholesterol from the plasma membrane before fertilizing potential is reached
Term
How long is an oocyte fertilizable in a cow? Is this shorter or longer than most livestock species?
Definition
Oocytes are fertilizable for up to 12 hrs post-ovulation. This is an short to average length of fertilizability in oocytes, but is shorter than the viable lifespan of the sperm
Term
Can cattle be inseminated with liquid and frozen sperm?
Definition
Yes
Term
What is the inseminating dose for cattle?
Definition
1 mL volume, with 6-8 mill sperm/dose
Term
What are the drawbacks to liquid semen, making frozen semen more preferred?
Definition
Liquid semen requires refrigeration and has a short storage life of about 4 days
Term
What are the benefits to frozen semen over liquid semen in cattle? What is the drawback?
Definition
Frozen semen has a indefinite storage time; goes through freeze/thaw very well but drawback is that it requires a liquid nitrogen freezer
Term
What is the volume of a standard French straw?
Definition
0.5 mL
Term
What is the volume of the modified french straw?
Definition
0.25 mL, same length as the standard french but 1/2 the diameter
Term
What is the volume of the continental straw?
Definition
0.25 mL; same diameter as the standard french but only have the length
Term
What are the advantages of the straw?
Definition
Increased post-thaw viability b/c straw shape allows for more even distribution of heat, lowers amount of exposure to heat b/c water bath is only 20-60 sec
Term
What is the conception rate after the 1st AI service?
Definition
60-70%
Term
What is the conception rate after the second AI service?
Definition
80%
Term
what is the conception rate after the use of clean up bull?
Definition
Up to 90%
Term
Summary for AI in cattle
Definition
Heat check 2x/day; use am/pm rule when breeding; 0.5mL dose with 6-8mill sperm/straw; 70% motility; 80%+ normal sperm; body temp for 20-60sec; rectovaginal technique; uterine body = site of deposition
Term
What is estrus?
Definition
Standing heat: a behavior induced by the presence of a large estrogen secreting follicle
Term
what is estrous?
Definition
reproductive cycle in females
Term
What is the most common way of detecting estrus in cattle? How successful is it?
Definition
Visual inspection; one of least successful b/c improperly trained personnel and frequency/timing cows show estrus (all day, only get checked at certain times)
Term
How is estrus detection commonly done in swine?
Definition
Allowing boar to mingle with the gilts/sows
Term
How long is estrus in gilts? Sows?
Definition
34-42 hours in gilts, 48-72 hours in sows
Term
What is the timing interval for inseminating swine?
Definition
12 and 24 hours post standing estrus (gilts); 24 and 36 hours post standing estrus (sows)
Term
what is the relation between weaning to estrus interval and duration of ovulation?
Definition
WEI is negatively related to duration of ovulation (longer WEI = shorter duration of estrus)
Term
What are the two major reasons for "customizing" the insemination protocol on a farm?
Definition
avoid wasting insemination doses and late insemination can have negative effects on pregnancy rate/litter size
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