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proved DNA is hereditary material |
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Substance of inheritance Genetic material located on chromosome A:T C:G |
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compared number and ratio of nitrogenous bases |
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Proved DNA was double helix nobel prize winners A-T: 2 hydrogen bonds C-G: 3 hydrogen bonds Purine + Pyrimidine = just right |
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Theories of DNA Replication: Conservative |
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parent molecule intact, new molecule of new nucleotides; not right |
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Theories of DNA Replication: Semiconservative |
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template model, 1 old 1 new; Right |
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Theories of DNA Replication:Dispersive |
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old and new DNA molecule mixed on all strands |
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to get from DNA to protein |
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in the nucleas DNA -> mRNA |
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in the cytoplasm, ribosomes mRNA -> Protein |
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triplet of nucleotides that code for a specific amino acid Start condon: AVG Stop condon: UAA, UAG, UGA Condon code is redundant not ambiguous |
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several things code for 1 amino acid |
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No each triplet codes for only one amino acid |
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no affect (GUG ~ GUA: makes same amino acid) |
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GU~ GCG causes a change in amino acid |
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stops whole process of translation turns into a stop codon (Nucleaisis corrects accidental change in existing DNA) |
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Step one: Starts in promoter region [Tata box] Step two: unwinds one strand becomes the template [Initiation] Step 3: Strand starts to [elongate] Step 4: [Termination] making new mRNA strand |
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Eukaryotes: adds 5' cap and a poly A tail b/c cytoplasm is dangerous Prokaryotes: goes straight into translation (it can do translation and transcription at the same time) |
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where mRNA becomes protein |
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1)E: T-RNA [exits] 2)P: [Polypeptide] chain grows 3)A: transfer RNA, TRNA [Arrives]
Step 1: Initiation- unwind, one strand becomes template Step 2: Elongation: Codon Recognition |
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keeps it from rewinding/overwinding before replication fork |
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Enzymes: Single Strand Banding Protein |
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tacks it up, stabalizing unwound DNA |
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Enzymes: DNA Polymerase 3 |
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adds nucleotides to new strand (light blue) |
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glues all together, fills the gaps, link okazaki fragments and DNA that replicate |
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removes the RNA chunks from RNA primer and replaces with DNA |
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1) DNA Helicase 2) Topoisomerase 3) Single Strand Binding Protein 4) Primase/ RNA Primer 5) DNA polymerase 3 6) DNA Ligase 7) DNA Polymerase I |
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-occurs in interphase (mitosis and meiosis) -2 DNA strands are antiparallel to each other -adding nucleotides to the 3' end [5' => 3'] -Eukaryote: 1 fork per bubble |
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Virus structure: Capsomere |
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Virus Structure: Viral Envelope |
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Ways virus can spread:Lytic cycle |
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feel symptons of being sick Virus replicates in cell and lysies (bursts) Host cell dies
virulent virus: only goes through lytic cycle |
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Ways a virus will spread: Lysogenic |
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Do not feel symptons Host cell lives
Temperate virvs: goes throug lytic and lysogenic cycle |
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Provirus: viral insertaion (ex: herpes) Retrvirus: goes from RNA-> DNA (quick mutation) Ex: HIV leading cause of death -Zambia highest infect rate |
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only infect plants Spread Horizontal: insects gardening tools Vertical: from parent |
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misfolded protein -replicate by contact Ex: mad cow disease (10 year incubation period) |
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proved the semiconservative model correct -medium use: ecoli |
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Needed for Hardy-Weinburg |
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large population no mutations random mating no migration no natural selection |
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