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2 Major Ways of Exploring Biology |
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1. Discovery Biology 2. Experimental Biology (Scientific Method) |
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2 Theories Define Biological Science |
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-Cell Theory -Theory of Evolution |
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-All living things are composed of cells -All cells come from pre-existing cells -Cells are the basic units of life |
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Robert Hooke- cellulae- dead cells
Leewenhoek-animalcules- sperm, pond water |
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Where Do Cells Come From? |
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Pre-existing cells -process called spontaneous generation |
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-Descent from a common ancestor (pattern) -Darwin observed these patterns -Modifications due to natural selection (survival of the fittest) |
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heritable traits that increase fitness (the attribute to survive conditions & reproduce) |
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Man chooses certain traits-used a lot in agriculture |
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BACTERIA
LUCA ARCHAEA
EUKARYA
Last Universal Common Ancestor |
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(Linnaeus) Domain Kingdom Phyllum Class Order Family Genus Species
Dumb King Phillip Can Order Free Grape Soda |
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Domain Bacteria & Domain Archaea |
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Prokaryotes (Prekernal) -No nucleus -No membraneous Organelles -Single Celled Organisms |
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Eukaryotes (True Kernal) -Have a nucleus -Single or multi-celled
(Protists, Fungi, Plants, Animals) |
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-cells -energy (can capture and use) -information (DNA, RNA) -replication (species can survive) -evolution (populations adapt) |
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Breaking down components, build back up |
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Atom Molecule Cell Tissue Organ Organ System Multi-celled organism Population Community Ecosystem Biosphere |
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Theory of Chemical Evolution |
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Abiotic Synthesis -One time spontaneous generation event |
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Building Blocks: Elements |
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-Fundamental basis of matter -Cannot be broken down -92 naturally occur -Each is composed by 1 atom type |
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Most Common Elements in Living Organisms |
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Hydrogen Carbon Nitrogen Oxygen
Make up 96% of all living things |
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Make up less that .01% of living things |
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What Determines Element Behavior? |
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-Smallest particle that retain properties of an element -Consists of subatomic particles Protons Electrons Neutrons |
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-Found in nucleus -1 amu -defines the atom - (+) POSITIVE CHARGE |
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-Found outside of nucleus -No measurable weight -Determines behavior - (-) NEGATIVE CHARGE |
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-Found in nucleus -1 amu -Defines isotope -NO CHARGE |
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To Be Electrically Neutral |
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# of protons ALWAYS = # of electrons |
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-Symbol -atomic number (# of protons) on bottom, NEVER CHANGES -mass number (# of protons + neutrons) CAN CHANGE |
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-atoms w/ variable # of neutrons -can make nucleus unstable because they emit energy particles |
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Electrons & Atom Behavior |
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-Determines if atoms will interact or bond -Based off of numbers and arrangements |
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-Hold electrons -electron shells
1st layer- max of 2 electrons 2nd, 3rd, 4th etc.- max of 8 electrons |
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-Valence Shell -Full= Stability (Inert & Un reactive) -Vacancies= Reactive (Not full) -Valence - number of unpaired electrons |
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-Fill electron vacancies -Covalent bonds (shared) -Ionic bonds (transfer) |
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MOST STABLE -atoms share a pair(s) of electrons to fill outermost shell -bonds can be single, double, or triple |
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Equal sharing of electrons |
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Unequal sharing of electrons - Due to electronegativity |
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one atom has greater pull on electron -leads to partial charge |
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-Molecular format -Structural formulas -Ball & Stick models -Space filling models |
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-atoms transfer electrons -changes proton; electron ratios |
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Charged atoms or molecules -cation (+) -anion (-) Unalike charges attract forming bond |
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2 or more atoms bonded in a given ratio |
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Sum of mass numbers of all atoms |
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Stable yet continual shift from forward to reverse |
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The sum of chemical reactions -how cells do work -requires energy and and aqueous solution |
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Stored -Chemical -stored in bonds |
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In motion -Thermal -molecular motion Measured in temp -Heat -uncontained energy Transferred thermal energy |
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1. Energy cannot be created or destroyed, only transformed or transferred
2. Natural tendency toward disorder or ENTROPY |
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Organisms Can Increase Order By... |
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cells combine chemical reactions -energy releasing reactions with energy requiring reactions |
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Release energy -spontaneous |
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Require energy input -non spontaneous |
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-essential to life -66% of body weight -exist in 3 physical states: solid, liquid, and gas |
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-Polarity -Hydrogen bonding -Solvent -Cohesion -Temperature stabilizing capabilities |
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Water is a Polar Covalent Molecule, Meaning.. |
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-no net charge -leads to hydrogen bonding |
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-weak bonds between polar molecules or polar molecules and ions. -break and reform easily -strong in high numbers |
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-dissociates many solutes into solution Hydrophilic or Hydrophobic |
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easily dissolved -ions and polar molecules |
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do not dissolve -non polar, water repels (i.e. oil) |
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Cohesive and Adhesive Properties |
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-water sticks to itself and other polar/ charged stuff -life is dependent on these properties -cohesion creates surface tension :water's property to resist breaking under pressure |
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Temperature Stabilizing Effects |
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-high capacity to absorb heat energy |
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amount of energy necessary to raise one gram of water one degrees Celsius -takes much energy to increase temperature |
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High Heat of Vaporization |
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-much energy to convert from liquid to a gas |
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Temperature Stabilization: ICE |
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less dense than liquid water causes it to float |
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Hydrogen power 0-14 Neutral at 7
[H+] using logarithmic- goes up or down by 10 times |
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compounds that minimize pH shifts |
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bonding capacity -covalent bonds -can bond w/ up to 4 atoms
leads to diverse molecular shapes |
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same molecular formula, but different structural formula |
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mirror images (L-Dopa & D-Dopa) |
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-covalently bonded atoms or clusters of atoms -gives carbon skeleton properties -participates in chemical reactions -gives molecular diversity |
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Stages of Chemical Evolution |
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1. Synthesis of organic molecules 2. Formation of "building blocks" :prebiotic soup 3. Polymerization of large molecules 4. Large molecules could replicate |
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-Large chain molecules consisting of smaller similar subunits (monomers) -Linking process is polymerization -Assembled and disassembled by 2 chemical reactions |
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1. Condensation reaction: monomer in, water out 2. Hydrolysis: water in, monomer out |
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involved in cell structure and function -structural; support -storage; storage of amino acids -transport; of other substances -hormonal; coordination of an organism's activities -receptor; response of cell to chemical stimuli -contractile; movement -defensive; protection against disease -enzymatic; selective acceleration of chemical reactions |
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-folded polymers of amino acids -diversity based on the arrangement of 20 amino acids |
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Contain: -Amine group -Carboxyl group -R group |
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-distinguishes each amino acid -gives each amino acid specific properties -determine reactivity and folding of polymer |
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Protein Synthesis: Polymerization |
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-Linking amino acid monomers together forming chains -Peptide bonds form between the monomers
-Oligopeptides- 50 or less -Polypeptides- 50 or more
-Upon interaction and folding ia a protein |
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-most diverse class of molecules for shape -form fits function -4 levels of organization (primary,secondary, tertiary, quaternary) |
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-Unique sequence of amino acids -controls final shapes -instructed by DNA -single amino acid change disrupts the final shape and function |
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involves hydrogen bonding between atoms of the polypeptide's backbone 2 basic structures -helix -pleated sheets |
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Binding between R groups form irregular contortions |
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collagen, tendons, ligaments |
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a combination of two or more polypeptides interacting to form a single structure |
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Dependent on the cellular enviornment -pH, temp, and salts Molecular chaperones can facilitate folding |
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-infectious proteins (virus-like) -proteins that are misfolded |
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-catalytic proteins- accelerate chemical reactions -end in -ase -one enzyme for one substrate (reactants) -not consumed or altered by reaction -maximize energy use |
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Chemical Reactions: Barriers |
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Energy barriers -prevent spontaneous breakdown of reactants
Overcome barriers -kinetic energy increases -reactants collide and bonds break/reform: transition state |
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-decrease energy input necessary to reach the transition state -lowers activation energy (energy required to initiate chemical reaction |
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Enzymes are Substrate Specific |
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-each type of cellular reaction is catalyzed by a specific enzyme |
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pocket for substrate binding -induced fit occurs upon binding -shape change of enzyme |
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Catalytic Cycle of an Enzyme |
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1. Initiation: Reactants bind for enzyme-substrate complex 2. Transition State Facilitation: enzyme induced fit lowers Ea 3. Termination: Products released, enzyme is unaltered |
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-inorganic metal ions -organic molecules-coenzymes |
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-affected by pH, temp, and salts -excess substrate |
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Competitive inhibition-something in the way Allosteric inhibition-shape changes -activation -deactivation |
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Nucleotides' 3 Components |
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-5 Carbon Sugar -Phosphate Group -Nitrogen Base |
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A-T,G-C Double Helix-strands run anti parallel |
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Occurs in 5 prime to 3 prime direction Joined by phosphodiester bonds |
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energy form phosphorylated molecules |
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1. Strands separate-hydrogen bonds break 2. Complementary base pairing to template 3. Polymerization- sugar phosphate backbone is sealed |
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Single polynucleotide strand carries out DNA instructions by complementary strands |
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-H-Bonding within a single strand -3 types: mRNA, tRNA, rRNA |
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