Term
| In a ______, a glass electrode/pipette is filled with fluid |
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Definition
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Term
| What question is studied through voltage-clamp recordings? |
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Definition
| how do cell channels/current react to changes in voltage? |
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Term
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Definition
| keep the voltage of the neuron at a set point by injecting current through solution in the pipette |
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Term
| How do current-clamp recordings relate to voltage-clamp recordings? |
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Definition
| applying a very similar process to voltage-clamp, but in the reverse |
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Term
| What happens if you put 0 nA of current into the cell in a current-clamp recording? |
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Definition
| the voltage will remain at the resting membrane potential |
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Term
| excitatory postsynaptic potential (EPSP) |
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Definition
| depolarizing the cell closer to an action potential |
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Term
| inhibitory postsynaptic potential (IPSP) |
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Definition
| hyperpolarizing the cell farther from an action potential |
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Term
| excitatory postsynaptic current (EPSC) |
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Definition
| current across the membrane is going down |
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Term
| inhibitory postsynaptic current (IPSC) |
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Definition
| current across the membrane is going up |
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Term
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Definition
| a way to look at a single channel on the cell membrane instead of a population of channels |
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Term
| In cell-attached patch-clamp recording configuration, what happens when the electrode touches the cell? |
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Definition
| a little bit of membrane bubbles into it |
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Term
| In cell-attached patch-clamp recording configuration, what is the electrode filled with? |
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Definition
| the electrode is touching the outside of the membrane so it must be filled with EXTERNAL solution (mimics the cerebral spinal fluid) |
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Term
| In whole-cell patch-clamp recording configuration, what happens when the electrode touches the cell? |
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Definition
| it will seal up the cell membrane around the electrode |
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Term
| In inside-out patch-clamp recording configuration, what happens when the electrode touches the cell? |
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Definition
| the bubble thing happens like cell-attached patch-clamp recording configuration, but you pull it off the cell! |
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Term
| What is the advantage of inside-out patch-clamp recording configuration? |
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Definition
| you can put a drug into the CSF & see how it would directly affect the inside of the cell & its channels & all that |
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Term
| In inside-out patch-clamp recording configuration, what is the electrode filled with? |
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Definition
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Term
| Outside-out patch-clamp recording configuration has an initial setup like the ______ configuration |
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Definition
| whole-cell patch-clamp recording (initially seals with membrane) |
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Term
| Similarity between outside-out patch-clamp recording configuration & inside-out patch-clamp recording configuration |
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Definition
| you pull the electrode away |
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Term
| outside-out patch-clamp recording configuration vs. inside-out patch-clamp recording configuration |
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Definition
| in outside-out patch-clamp recording configuration, the outside of the membrane is facing the CSF |
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Term
| When recording currents through a single ion channel, what does a flat line indicate? |
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Definition
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Term
| When recording currents through a single sodium channel, what does it look like when the channel is open? |
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Definition
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Term
| When recording currents through a single potassium channel, what does it look like when the channel is open? |
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Definition
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Term
| Is it possible to isolate & record a single channel? |
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Definition
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Term
| Are channels triggered to open immediately after a voltage change? |
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Definition
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Term
| When you average the opening & closing of all channels of a certain ion, what does the graph look like? |
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Definition
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