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cell cyle and DNA replication
powerpoint 23
15
Biology
Undergraduate 1
04/20/2012

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Term
DNA replication
Definition
the hydrogen bonds of parent strands are separated. the two strands are used as a template to make daughter strands
Term
Semi Conservative
Definition
each daughter molecule gets half of the parent molecule
Term
Origin of replication
Definition
where replication begins. there are thousands on each chromosome
Term
Rate of replication
Definition
2000 bp/minute
Term
Origin Replication Complex
Definition
Binds at origin to initiate replication. can only work 5 prime to 3 prime
Term
Okazaki Fragments
Definition
Fragments of the 3 prime 5 prime strand
Term
leading strand
Definition
5 prime 3 prime strand that stays whole during replication
Term
DNA Polymerace
Definition
has the ability to correct its errors but putting the correct base into the sequence
Term
First step in DNA replication
Definition
The indicator protein binds to the Dna at the Origin of replication and begins to unwind the strands
Term
Step two of DNA Replication
Definition
Helices continue to unwind the DNA and DNA Gyrase promotes DNA seperation. You now have a replication fork
Term
Step three of DNA Replication
Definition
Primase binds to the first priming sequence on leading strand.It makes a short RNA primer that is complementary to the DNA template
Term
Step four of DNA Replication
Definition
DNA Polymerace III initiates DNA synthesis by adding deoxyribonucliotides to the 3 prime end.
Term
Step Five of DNA Replication
Definition
An RNA primer is made for the lagging strand. DNA polymerase extends the strand
Term
Step Six of DNA Replication
Definition
A Series of Rna Primers is needed to synthesize the lagging strand because it is discontinuous. DNA is synthesized at the 3 prime end of each primer making an Okazaki fragment that grows untill it meets an adjacent fragment. RNA primer is removed by the 5 prime exonuclease and replaces with DNA by the polymerase of the same enzyme
Term
Step Seven of DNA replication
Definition
DNa Ligase links together with adjacent Okazaki fragments with covalent phosphodiester bonds.
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