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chapter 5 metabolism
for test 2
30
Biology
Undergraduate 3
04/04/2011

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Term
siderophores
Definition
iron-binding agents used by cells to obtain iron in its insoluble ferric form (Fe3+)
Term
selective media
Definition
inhibits growth of some microorganisms but not others
Term
What is MacConkey agar made up of?
Definition
Bile salt, peptone and lactose
Term
How is MacConkey agar selective?
How is it differential?
Definition
selective - gram positive cannot grow in it, gram negative can
Differential - all cells with a pH<7 turn pink from lactic acid fermentation
Term
differential media
Definition
contains dye that detects particular chemical rxns that occur during growth
Term
sterilation
Definition
absence of all microorganisms
Term
exergonic
Definition
energy is released and products have lower energy than reactants
Term
what kind of bonds are between enzymes and their substrates?
Definition
weak bonds: vaan der walls, hydrogen bonds, and hydrophobic interactions
Term
electron acceptors have a _____positive reduction potential
Definition
more
Term
when a substance is _______, is accepts an electron. When a substance is _______, it donates an electron.
Definition
reduced, oxidized
Term
the two types of phosphorilation that produce energy are...
Definition
fermentation (substrate-level) and respiration (oxidative)
Term
what are the products of ethanol fermentations?
Definition
2 ATP
2 CO2
2 ethanol
Term
what are the products of lactate fermentation?
Definition
2 ATP
2 Lactate molecules
Term
what two substances can be used as primary donors for Electron Transport Systems in aerobic respiration?
Definition
NADH and FADH2
Term
name the 5 oxidation-reduction enzymes in the electron transport system in the order that they occur.
Definition
NADH dehydrogenase, flavoprotein, cytochrome, iron-suler protein, quinone
Term
which of the ETS enzymes donates/passes on both electrons and protons?
Definition
NADH dehydrogenases
Term
which of the ETS enzymes is not a protein?
Definition
quinone
Term
what is ATPase?
Definition
an enzyme that converts the PMF into ATP using F1 and F0, its protein components
Term
which of ATPase's protein components faces the cytoplasm?
Definition
F1
Term
respiration using FADH produces ___ ATP and respiration using NADH produces ___ ATP
Definition
2, 3
Term
what is the precursor for the biosynthesis of glycogen?
Definition
ADPG
Term
what is theprecursor for glucoses needed for polysaccharides?
Definition
UDPG
Term
What is PEP synthesized from?
Definition
oxalacetate, a CAC intermediate
Term
The beginning and end products of ____________ are PEP to glucose
Definition
gluconeogenesis
Term
what enzymes obtain nitrogen from the environment to be used fo the formation of amino groups in amino acids?
Definition
Glutamate dehydrogenase and glutamine synthetase
Term
what is the job of Acyl Carrier Proteins, ACPs?
Definition
synthesize fatty acids two carbons at a time, hold them as theyre being synthesized
Term
what enzymes obtain nitrogen from the environment to be used fo the formation of amino groups in amino acids?
Definition
Glutamate dehydrogenase and glutamine synthetase
Term
define allosteric enzyme
Definition
is the first enzyme in a biosynthetic chain, has 2 sites for binding - one for the substrate and one for the end product.
Term
define allosteric enzyme
Definition
is the first enzyme in a biosynthetic chain, has 2 sites for binding - one for the substrate and one for the end product.
Term
besides feedback inhibition, some enzymes are regulated by covalent modifications. name 4 common modifiers.
Definition
AMP, ADP, PO4, CH3
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