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Chemistry Internship Study Questions Section 1
Lipids, NPN, & Bilirubin
83
Chemistry
Undergraduate 4
10/23/2015

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Cards

Term
Fill in the chart, For "highest" levels, list the most common conditions:
A
[image]
Definition
Protein
Term
Fill in the chart, For "highest" levels, list the most common conditions:
B
[image]
Definition
Muscle
Term
Fill in the chart, For "highest" levels, list the most common conditions:
C
[image]
Definition
Purine
Term
Fill in the chart, For "highest" levels, list the most common conditions:
D
[image]
Definition
Renal Disease, Heart Failure, Shock
Term
Fill in the chart, For "highest" levels, list the most common conditions:
E
[image]
Definition
Renal Disease, Muscle Disease
Term
Fill in the chart, For "highest" levels, list the most common conditions:
F
[image]
Definition
Gout, Leukemia, Chemotherapy
Term
Fill in the chart, For "highest" levels, list the most common conditions:
G
[image]
Definition
6-20 mg/dL
Term
Fill in the chart, For "highest" levels, list the most common conditions:
H
[image]
Definition
0.5-1.1 mg/dL
Term
Fill in the chart, For "highest" levels, list the most common conditions:
I
[image]
Definition
3.0-8.5 mg/dL
Term
Is conjugated Bilirubin Direct or Indirect?
Definition
Direct
Term
Is unconjugated Bilirubin Direct or Indirect?
Definition
Indirect
Term
Is conjugated Bilirubin water soluble?
Definition
Yes
Term
Is unconjugated Bilirubin water soluble?
Definition
No
Term
Is conjugated Bilirubin Detectable in Urine?
Definition
Yes
Term
Is unconjugated Bilirubin Detectable in Urine?
Definition
No
Term
Is conjugated Bilirubin Albumin-Bound?
Definition
No
Term
Is unconjugated Bilirubin Albumin-Bound?
Definition
Yes
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
A
[image]
Definition
Hemolytic Disorders, Hemolytic Disease of the Newborn, Transfusion Reactions
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
B
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
C
[image]
Definition
Normal
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
D
[image]
Definition
Negative
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
E
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
F
[image]
Definition
Liver Disease, Hepatitis, Cirrhosis
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
G
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
H
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
I
[image]
Definition
Positive
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
J
[image]
Definition
Increased OR Normal
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
K
[image]
Definition
Bile duct obstruction from gall stones, Tumors
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
L
[image]
Definition
Normal
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
M
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
N
[image]
Definition
Increased
Term
Fill in the chart, indicate if levels will be typically normal, increased, or decreased:
O
[image]
Definition
Decreased
Term
List the reference ranges (including reporting units) for:
Total Cholesterol
Definition
<200 mg/dL
Term
List the reference ranges (including reporting units) for:
Triglyceride
Definition
<150 mg/dL
Term
List the reference ranges (including reporting units) for:
HDL
Definition
>60 mg/dL
Term
List the reference ranges (including reporting units) for:
LDL
Definition
<110 mg/dL
Term
Why would it be acceptable to run total cholesterol on a random sample versus a fasting sample?
Definition
Most cholesterol is produced by the liver.
Only 10-15% comes from dietary intake.
Term
Describe the appearance of a serum sample collected after a meal and allowed to sit refrigerated overnight.
Definition
Clear with milky top layer
Term
What lipoprotein is consistent with a serum sample that appears clear with a milky top later after a meal and has been allowed to sit refrigerated overnight?
Definition
Chylomicrons
Term
Match the lipid with its carrier protein (lipoprotein):
VLDL
Definition
ENDOgenous Triglyceride
Term
Match the lipid with its carrier protein (lipoprotein):
LDL
Definition
Transports Cholesterol TO Tissues
Term
Match the lipid with its carrier protein (lipoprotein):
HDL
Definition
Transports Cholesterol AWAY FROM Tissues
Term
Match the lipid with its carrier protein (lipoprotein):
Chylomicrons
Definition
EXOgenous Triglyceride
Term
Match the lipid with its carrier protein (lipoprotein):
Endogenous Triglyceride
Definition
VLDL
Term
Match the lipid with its carrier protein (lipoprotein):
Transports Cholesterol TO Tissues
Definition
LDL
Term
Match the lipid with its carrier protein (lipoprotein):
Transports Cholesterol AWAY FROM Tissues
Definition
HDL
Term
Match the lipid with its carrier protein (lipoprotein):
EXOgenous Triglyceride
Definition
Chylomicrons
Term
What genetically controlled lipoprotein is responsible for coronary heard disease at an early age when detected in high amounts?
Definition
Lp (a)
Term
Give the manual calculation for VLDL
Definition
Triglycerides / 5
Term
Give the manual calculation for LDL
Definition
Total - HDL - (Triglycerides/5)
Term
Under what situation are the VLDL & LDL calculations invalid?
Definition
When triglycerides are >400
Term
The enzymatic method for triglyceride quantitation involves measuring liberated glycerol. What is the enzyme used in this method?
Definition
Lipase
Term
List the reference range for total bilirubin in a normal adult
Definition
0.1-1.2 mg/dL
Term
List the reference range for direct bilirubin in a normal adult
Definition
0-0.5 mg/dL
Term
How is an indirect bilirubin calculated?
Definition
Total - Direct
Term
Describe any specimen handling requirements for bilirubin if testing is delayed
Definition
Avoid light exposure (may cause false negative)
Term
What is the name of the reagent used in the classic bilirubin reaction?
Definition
Diazo
Term
How will hemolysis in the sample affect bilirubin results?
Definition
Decrease Value
Term
How will lipemia in the sample affect bilirubin results?
Definition
Increase Value
Term
What is the purpose of adding an accelerant to a bilirubin reaction?
Definition
Converts Indirect to Direct so that all bilirubin (total) is measured
Term
What is the accelerant used in the Malloy-Evelyn method for total bilirubin?
Definition
Methanol
Term
What is the accelerant used in the Jendrassik-Grof method for total bilirubin?
Definition
Caffeine-benzoate
Term
When can determination of bilirubin by direct spectrophotometry be used?
Definition
Newborns only (x<1 month).
Read absorbance @ 450 nm
Term
After birth, at what age will a newborn's bilirubin level equal normal adult levels?
Definition
1 Month
Term
What is the formula to find the corrected CrCl?
Definition
Observed Creatinine Clearance * (1.73 / Body Surface Area)
Term
What is the formula to find the observed CrCl?
Definition
(Urine Creatinine *Urine volume) / (Serum Creatinine * Time (min))
Term
Why must sodium fluoride tubes be avoided when measuring BUN & Creatinine?
Definition
Causes Falsely Decreased BUN
Term
What body function is being assessed by the creatinine clearance?
Definition
Glomerular Filtration Rate
Term
What is the normal average Creatinine Clearance for an adult?
Definition
120 mLs/min (on average)
Term
Calculate the Observed CrCl results for the given set of data:
Urine Creatinine = 100 mg/dL
Serum Creatinine = 1.1 mg/dL
Collection Time = 12 hours
Urine Volume = 655 mLs
Body Surface Area = 2.34 m2
Definition
83 mLs/Minute
Term
Calculate the Observed CrCl results for the given set of data:
Urine Creatinine = 56 mg/dL
Serum Creatinine = 1.2 mg/dL
Collection Time = 24 Hours
Urine Volume = 1 Liter
Body Surface Area = 1.6 m2
Definition
32 mLs/Minute
Term
Calculate the Corrected CrCl results for the given set of data:
Urine Creatinine = 100 mg/dL
Serum Creatinine = 1.1 mg/dL
Collection Time = 12 hours
Urine Volume = 655 mLs
Body Surface Area = 2.34 m2
Definition
61 mLs/Minute
Term
Calculate the Corrected CrCl results for the given set of data:
Urine Creatinine = 56 mg/dL
Serum Creatinine = 1.2 mg/dL
Collection Time = 24 Hours
Urine Volume = 1 Liter
Body Surface Area = 1.6 m2
Definition
35 mLs/Minute
Term
What is the name of the classic reaction for creatinine?
Definition
Jaffe
Term
What is the primary reagents used for the Jaffe method (classic reaction for creatinine)?
Definition
Alkaline Picrate
Term
What is an eGFR?
Definition
Estimated Glomerular Filtration Rate
Term
What is the benefit of performing an eGFR over a traditional CrCl?
Definition
No need for urine decreased levels associated with chronic kidney disease. Calculated from serum creatinine, body weight, height, sex, and race.
Term
Why may the Cystatin C test be preferred over a creatinine clearance for assessment of renal function?
Definition
Formed at a constant rate.
No urine needed.
A decreased GFR will cause a Increase in the Serum.
Term
What is a normal BUN:Cr ratio?
Definition
10:1 - 20:1
Term
What is the purpose/use of calculating a BUN:Cr ratio?
Definition
May help differentiate pre-renal from renal causes of an Increased BUN
Term
High ammonia levels are consistent with what organ failure?
Definition
Liver
Term
What disease is associated with high ammonia and encephalopathy in children who have taken aspirin to treat flu symptoms?
Definition
Reye's Syndrome
Term
What type of tube and special handling is required when collecting samples for ammonia?
Definition
Lithium heparin tubes are preferred.
EDTA tubes are OKAY.
Place on Ice!
Separate ASAP & Freeze up to 24 Hours
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