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Definition
involves determining the rate at which enzymes move through a gel when subjected to an electrical field. |
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Term
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Involves directly determining the sequence of nucleotides in a segment of DNA |
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Restriction Fragment Length Polymorphism |
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Definition
DNA is cut using a restriction enzyme and the electropheresis is used to determine the length |
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Definition
Identifying repeating sequences of nucleotides and comparing the lengths of those sequences |
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Definition
the proportion of genes that are polymorphic |
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The frequency of the most common allele is less than some arbitrary threshold usually 95-99 percent |
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Definition
proportion of genes at which the average individual is heterozygous |
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levels of genetic diversity |
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Definition
among higher taxa, species and subspecies populations and individuals |
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2 uses of heterozygosity values |
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Definition
the first use is to compare the observed heterozygosity to the expected heterozygosity the second use is to determine how much genetic diversity is within populations vs how much is between populations |
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Hardy-weinburg assumptions |
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Definition
population is sexually reproducing non overlapping generations, no mutations, no migrations, no selection, infinite population, random mating |
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positive assortative mating |
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Definition
individuals are more likely to make with like individuals which produces more homozygotes |
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Negative assortative mating |
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Definition
mate with unlike individuals and produce heterozygotes |
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Definition
H(t) = H(s) + D (st) H(t) is total heterozygosity H(s) heterozygosity within populations D(st) heterozygosity between populations |
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Definition
sampling error in the transmission of alleles from generation to generation |
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Measuring the effect of genetic drift |
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Definition
greater population equals less impact from genetic drift |
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examples of bottlenecking |
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Definition
cheetahs, northern elephant seal |
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Definition
high mortality of young, high proportion of defective sperm, inbreeding loss of rare alleles |
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Northern Elephant Seal 8==D |
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Definition
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new population originates from smaller founder population, same effects as bottleneck |
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Why is inbreeding so dangerous |
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Definition
harmful recessive alleles are more likely to become homozygous meaning theyll be expressed |
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effective population size |
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Definition
the number of individuals contributing genes to the next generation, effective population is less than census population |
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Definition
Hardy weinburg assumptions, effective population = ideal population with as much drift as actual population |
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Factors that affect genetic diversity |
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Definition
variation in population size, unequal sex ratio |
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Definition
applied studies of the behavior of alleles in population |
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