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Definition
| A double-stranded DNA copy of an mRNA molecule. |
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Definition
| A set of many copies of a DNA molecule. |
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Definition
| A large collection of DNA clones representing a particular population of DNA molecules. |
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Term
| Functional complementation |
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Definition
| Transformation of a mutant to wild type by a clone in a DNA library, allowing the clone carrying a gene of interest to be identified. |
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Definition
| The correction of a genetic deficiency in a cell or organism by addition and insertion into the genome of a normal gene. |
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Definition
| Use of recombinant DNA technology and transgenesis to alter the genetic machinery of an organism for human purposes. |
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Term
| Polymerase chain reaction (PCR) |
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Definition
| A method for amplifying a specific DNA fragment in vitro using repeated rounds of DNA synthesis. |
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Definition
| Cloning of a gene based on its location on a chromosome map. |
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Definition
| A molecule that recognizes and binds to a specific nucleic acid or polypeptide, allowing it to be identified in a complex mixture. |
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Definition
| A novel DNA molecule formed by the combination of two nonhomologous molecules, often from two different organisms. |
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Definition
| An enzyme that cleaves DNA at a specific sequence, in many cases producing complementary, "sticky" ends. |
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Term
| Restriction fragment length polymorphism (RFLP) |
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Definition
| Differences in the length of DNA fragments produced by differences in restriction enzyme cleavage sites between individuals. |
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Term
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Definition
| Determination of function of a cloned gene by creating a mutation in vitro and examining its phenotype in vivo. |
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Definition
| An enzyme that makes DNA (cDNA) from an RNA template. |
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Term
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Definition
| Replacement of a specific functional gene with a nonfunctional copy in order to assess the function of the normal gene. |
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Term
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Definition
| Introduction of a foreign gene or recombinant DNA molecule into an organism. |
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Term
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Definition
| In gene cloning, a DNA molecule that can carry a foreign piece of DNA and can be replicated in a host cell. |
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