Term
| What do restriction endonucleases do? |
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Definition
| Restriction Endocucleases are used to fragment DNA molecules at specific sites. |
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Term
| Restriction Endonucleases are useful for... |
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Definition
- forming a physical mapping of DNA
- allows for the creation of recombinant DNA
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Term
| How many bases can Type II restriction enzymes recognize and in what direction do they read it? |
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Definition
| Type II restriction enzymes can read 4-12 bases and read in the direction of 5'-3' |
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Term
| What are the products of cleaving DNA sequences? |
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Definition
| The products are "sticky ends" |
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Term
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Definition
| DNA ligase joins two DNA fragments to form a stable piece of DNA |
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Term
| How does Gel elctrophloresis seperate DNA fragments and in what direction does each move? |
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Definition
| It seperates them bases on size and the smaller fragments move farther away. |
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Term
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Definition
| When foreign DNA is introduced into cells. |
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