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Dr. Bechhofer's Fun House 1
Overview of Gene Expression
17
Biochemistry
Graduate
09/24/2010

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Cards

Term
Transcription
Definition
DNA-->mRNA
Term
Translation
Definition
mRNA-->protein
Term
Reverse Transcription
Definition
RNA-->cDNA-->mRNA-->Protein
Term
Pyrimidines
Definition
Cytosine, Uracil, Thymine
Term
Purines
Definition
Adenine, Guanine
Term
Nucleoside
Definition
Base plus a sugar
Term
Nucleotide
Definition
Base, sugar, phosphate
Term
Which is bigger? pyrimidines or purines?
Definition
Purines are bigger. They have 2 rings.
Term
What is the difference between RNA and DNA?
Definition
DNA has one of the OH groups removed
Term
What's the difference between a standard nucleotide and ATP, GTP, CTP, UTP, and TTP?
Definition
The others are triphosphates. Add 2 phosphate groups.
Term
Draw a nucleotide, and number it.
Definition
Look up a picture online to double check.
Term
What kind of bond do nucleotides make in DNA? Draw it.
Definition
5' to 3' phosphodiester bond. The phosphate of each nucleotide forms a bond with the 3' carbon of the next nucleotide. Look at a picture to confirm.
Term
How are two strands of DNA held together? Is this a type of covalent bond?
Definition
hydrogen bonds are not covalent
Term
What bonds together in DNA? (as in name the base pairings). Which ones are pyrimidines and which ones are purines?
Definition
G and C. A and T. C and T are pyrimidines. Adenine and Guanine are purines.
Term
How many H-bonds between G and C? How many between T and A?
Definition
3 between G and C. 2 between T and A.
Term
What kind of overall charge does DNA have?
Definition
Very negative
Term
What is hydrophilic in DNA? What is hydrophobic? What will be on the outside and on the inside?
Definition
charged phosphates are hydrophilic and on the outside. hydrophobic bases are on the inside?
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