Term
| Key factor in det rate of gene expression? |
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Definition
TX
How often Rna pol starts TX dictates how often hene is translated
Energy of conservation |
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Term
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Definition
Histone Acetyl Transferase
acetylates lyisne of dna which is wrapped around histone. lt looses its charge and falls off. Facilitates bromodomain Bind Proteins to bind nucleosome-->blocks association of dna w/ histone-->incr TX |
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Term
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Definition
opposite of HAts
slows Tx |
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Term
| Methylation of cpG islands |
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Definition
usually on GC rich regionsof 5' cpg islands--> dec in tx.
5' region usually site of regulation.
Methylation bocks TF from binding DNA |
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Term
| Affect of Methylation of cpg binding domain proteins |
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Definition
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Term
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Definition
target for anticancer drungs
in cancer hypermethylation of cpg--> tx silencing |
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Term
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Definition
The first nucleotide to be transcribed from the 3` end of the DNA sequence, at
the 5` end of the promoter region, before the Shine-Dalgarno sequence in
prokaryotes. Usually a T or C |
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Term
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Definition
| occur in non coding regions within promoter region |
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Term
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Definition
| occurs in proteins which bind dna regualtory sites and affect TX |
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Term
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Definition
| bind proximal to respnse elements ie CAAT box |
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Term
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Definition
bind TATA box
TF IID is first to bind
Then A, B and H=helicase
followed by RNA pol II which must be phsophorylated by H initiate TX |
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Term
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Definition
| seqs of dna distal intitiation site. req protein binding which loop and stabilize DNA for TX |
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Term
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Definition
U1 binds gu
u2 bind A in middle
U5 joins exon1 and 2 |
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Term
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Definition
RNA transport protein
transport through NPC
Facilitated transport |
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Term
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Definition
@ high [Fe] IRE cannot bind to mRNA stem loop structures --> degradation of mRNA
@ low [Fe] it can bind and protect stem loop
@ low[Fe] --> translation is possible
Fe can be transported out of the cell |
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Term
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Definition
| inhibit eEF-2--> no translation |
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Term
| effect of Picorna Virus, Polio, Rhina EMCV |
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Definition
eIF4E is reqd for 7 meth guan cap
Picorna has a 5' internal ribosomal entry site which bypasses normal controls for ribosomal assembly |
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Term
| Most common post translation MOD |
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Definition
phosphorylation of SEr
also glycosylation and phosphorylation of above + Thr and Tyr
Phosphorylation of Tyr for signal transduction |
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Term
| Post translational glycosylation |
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Definition
N linked on Asn req interm
O linked on Ser or Thy in Golgi no interm |
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Term
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Definition
| signal for protein to go to extra cellular space or compartamentilztion |
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Term
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Definition
starting block for branched chain glycosylation
occurs in golgi |
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Term
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Definition
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Term
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Definition
| E1, E2, E3 tag proteins for ubiquination |
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Term
| Ubiquination and Neurodeg disaease |
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Definition
| Damaged DNA makes RNA pol II bind to it Differently --> tagging of pol II with ubiquitin --> no TX |
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Term
| Effect of MODS on small RNA |
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Definition
| major effect on gene expression |
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