Term
| Maximum resolving power of light microscope? |
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Definition
-0.2 µm
-A RBC is about 6-8µm |
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Term
| Name two fixatives and what they react with? |
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Definition
-Formaldehyde and glutaraldehyde
-react with amine groups |
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Term
| Steps for embedding? Two types and advantages? |
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Definition
-Dehydration by bathing with a graded series of ethanol mixtures (from about 70-100%)
-Then ethanol is replaced by solvent miscible with embedding medium
-Then we embed with either paraffin or resin
-Resin can be run at a lower temp using a plastic solvent and so is subject to less shrinking |
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Term
| Phase-contrast microscopy? |
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Definition
-Relies on fact that light changes "phase" when going through a medium; peaks change position
-allows study of live organisms by avoiding dyes
-interference microscopy uses a similar method |
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Term
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Definition
| -uses polarized light to illuminate only structures with oriented molecules like cellulose, collagen, etc. |
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Term
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Definition
| -Thin plane of light is moved over a specimen and a computer reconstructs a surface map |
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Term
| Fluorescence microscopy? What is the dye? |
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Definition
| -Use acridine orange which complexes with DNA and RNA becoming yellow with DNA and reddish orange with RNA (stains cytoplasm) |
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