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Final
final study cue cards
242
Biology
Undergraduate 2
04/16/2014

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Term
Causes of different meat types?
Definition
-genetics
-muscle glycogen
-management from farm to packaging plant(stress)
Term
Why does pH decline post-mortem?
Definition
due to lactic acid build up
Term
pH in DFD meat in pork after 1 hour and after 24 hour?
Definition
1 hour: minimum drop in pH due to the fact they had low glycogen at slaughter so much substrate for lactic acid production
24h- pH still stays at high pH levels
Term
pH decline in normal pork:
-1h
-6-8h
-24 h
Definition
1-pH>6
6-8- pH of 5.6-5.7
24h- pH of 5.3-5.7
Term
P1 Pale PSE pork pH after 1 hour and 24 hour
Definition
1h-post-mortem see a rapid drop in pH(pH<6)
24h- range of 5.3-5.6(close to normal pork)
Term
P2 Pale PSE pork pH after 1, 6/8 and 24h
Definition
1-pH>6
6/8-pH<5.5
24-very low pH of 5.2-5.4
Term
4 physiological adjustments the animal makes to adverse conditions(aka stressors)
Definition
heart rate
respiration
body temperature
blood pressure
Term
fxn of coricoids
Definition
synthesis of carbohydrates maintenance of cell response and ion balance
Term
fxn epinephrine
Definition
maintenance of circulation; break down of glycogen and lipids
Term
fxn of the thyroid hormone
Definition
stimulation of oxidative metabolism
Term
What is more stressful: direct to the packer or auction?
Definition
auction
Term
how long can cattle legally be on a truck for?
Definition
53 hours
Term
How does co-mingling affect stress?
Definition
no social hierarchy as they are "strangers", this often leads to re-establisment of hierarchy which leads to fighting which decreases glycogen
Term
How does stress affect circulation?
Definition
Increases circulation due to increased nutrient and oxygen demand needed for muscle contraction
Term
What happens if body doesn't regulate stress?
Definition
muscle shifts to anaerobic metabolism and can't regulate the temperature(leads to hyperthermia). If lactic acid builds up it can be used by the liver and heart but too much causes systemic acidosis
Term
What are stress resistent animals and what is their down side?
Definition
Can handle exposure to stress and maintain temp and homeostasis HOWEVER at the expense of muscle glycogen which is an issue if the animal is not aloud to rest before slaughter
Term
What is Porcine Stress Syndrome?
Definition
caused by the interaction of biological stressors(genetics) and an environmental factor(pre slaughter stress)
Term
What is the name of the autosomal recessive gene(of pigs) in the human form?
Definition
malignant hypothermia
Term
What change is found in the body with malignant hypothermia?
Definition
a single nucleotide is replaced in Ca release channel protein of the SR causing excessive release of Ca for the SR, leading to continual stimulation of muscle contraction
Term
What is Ryanodine?
Definition
Plant alkaloid used to test for the PSS defect
Term
PSS pork can lead to what kind of meat?
Definition
Normal, PSE and DFD
Term
PSS pigs have a sensitivity to?
Definition
Halothane(anesthetic)which increases body temp, lactate production, muscle regitiy,major disturbances in water and electrolytes and affects RED AND WHITE MUSCLE
Term
What are the 3 classes of PSS?
Definition
NN-halthane resistant
Nn-carrier
nn-affected
Term
Circulatory failure in PSS pigs can arise due to?
Definition
-vasoconstriction
-hypertension
-decreased cardiac output
Term
What is hyperkalemia?
Definition
increased potassium levels in the blood in PSS pork in reaction to stress
Term
What type of pig breeds are more prone to PSS?
Definition
breeds associated with leaness ex pietrain, poland, china, landrace
Term
PSE stands for?
Definition
pale soft and exudative
Term
Why is blast chilling now used in PSE pork?
Definition
to prevent muscle pH from dropping before heat dissipated protein denaturation can occur
Term
if the animal has a pH of ____ after the first hour its a good inicator of PSE pork
Definition
pH<6
Term
where is PSE pork mainly found?
Definition
in the longissimus or the loin
Term
What is the Rendement Napole gene?
Definition
affects hampshires usually
causes high glycogen at slaughter leading to high lactic acid
Term
How does protein denaturation affect pigment?
Definition
loss of intensity-gives paleness to pigs who do NOT have much myoglobin to begin with
Term
give the relationship between water holding capacity and protein denaturation
Definition
lowers water holding capacity, making meat appear wet
Term
Does protein denatured pork hold brine well?
Definition
no
Term
What does DFD stand for?
Definition
dark firm and dry
Term
How do we get DFD from a PSS pork?
Definition
animal survives stress at cost of glycogen stores, leaving little to form lactate keeping a high pH
Term
Does DFD pork have a good water holding capacity?
Definition
yes
Term
Is PSE the same as PSS?
Definition
no
Term
What is water absorption?
Definition
the ability to bind water from external environment
Term
water holding capacity?
Definition
the ability to bind or hold own water
Term
True or False
water holding capacity is influenced by pH
Definition
TRUE! as pH decreases WHC decreases
Term
what happens to the thick and thin filaments postmortem?
Definition
they move closer decreases the water space between myofilaments
Term
Where does water escape through and why?
Definition
no energy for membranes to hold water
escapes through FASCICULI
Term
What does normal water loss contribute to in the muscle?
Definition
softness and easily separated muscle fibre bundles
Term
As WHC ____ drip loss ____
Definition
decreases, increases
Term
What fibers are most affected by DFD?
Definition
red fibers
Term
where does most rapid glycogen depletion take place?
Definition
in fast twitch or white muscle
Term
How much drip loss is there when there is max water holding ability?
Definition
none
Term
Why is DFD meat firm to the touch?
Definition
little protein denaturation in DFD meat
Term
Who has lower incidenses of DFD heifers or steers?
Definition
steers
Term
What are heifers given to prevent DFD beef?
Definition
MGA which stops from cycling, reducing the glycogen loss associated with estrus mounting behaviour and allowing more nutrients for gain
Term
what type of feed produces higher incidences of DFD beef?
Definition
forage finished beef
grains have more available energy
Term
what is the major reason for DFD beef?
Definition
mixing of cattle-no hieracrchy
Term
colour of the meat is influenced by?
Definition
viewing conditions and individual vvariation on ability to detect colour as well as overall structure and texture of muscle
Term
Appearance of meat is associated with?
Definition
concentration and state of heme pigments
Term
______ makes up 80-90% of total pigment in meat depending on how well the animal is bled out
Definition
myglobin
Term
how does cytochrome oxidase aid in appearance of meat?
Definition
includes a heme unit in it's structure
Term
why do milk fed veal calves have low myoglobin?
Definition
milk has low iron content
Term
Why does myoglobin in muscle increase with age?
Definition
as animal ages myoglobin loses affinity to bind oxygen and therefore needs more myoglobin in the muscle to get enough oxygen supply
Term
What is myoglobin content like in intacts vs castrates or females?
Definition
intact males have more myoglobin
Term
What colour is myoglobin?
Definition
purple in "fresh" cut meat referred to as deoxymyoglobin
Term
If the muscle is cut(between the 12th/13th rib) and allowed to bloom what will form?
Definition
oxymyoglobin
Term
what is oxymyoglobin stability based on?
Definition
oxygen supply
Term
What is required before beef grading can occur?
Definition
bloom period
Term
What form of myoglobin is formed when there is small amounts of oxygen available?
Definition
metmyoglobin, ferrous oxidized to ferric
Term
Is metmyoglobin formation post mortem aging?
Definition
NO
Term
True or false
nutrition of the meat is affected by change from oxymyoglobin to met
Definition
no!!
Term
How can surface browning be reduced
Definition
keep meat near freezing to minimize enzyme activity and oxygen utilization
Term
What 3 compounds are lethal when bound to hemoglobin?
Definition
CO, CN or nitrite
Term
as pH ____ light scattering ____
Definition
decreases, increases
Term
as sarcomere length ____ light scattering ____
Definition
decreases increases
Term
in regards to light reflectance why is PSE meat pale?
Definition
large proportions of free water increase reflectance and decrease overall light absorption, increasing paleness
Term
in regards to light reflectance why is DFD meat dark?
Definition
DFD has a high WHC due to high concentrations of intracellular water, this leads to minimal light reflection and increased absorption
Term
What is the most abundant protein in the body?
Definition
collagen
Term
Where is collagen found?
Definition
tendons
ligaments(bone-bone)
bones
cartilage
muscle
skin
blood vessels
Term
how does collagen help with muscle shape and form?
Definition
holds muscle fibers together(perimysium) and protects muscle fibers from being damaged from strong muscle contractions
Term
how does collagen aid in contractile force transmission?
Definition
when muscle contracts the contractile force is transmitted via CT sheaths to the tendon and then the bone
Term
Increased Connective tissue leads to?
Definition
tough meat
Term
Is collagen visible to the naked eye?
Definition
yes
Term
What makes the gristle in meat?
Definition
collagen
Term
True or False
Is connective tissue found in myofilaments?
Definition
false
Term
What is tropocollagen?
Definition
the structural unit of collagen fibril that is synthesized in the fibroblasts from amino acids which are typically non-essential
Term
Collagen and elastin are ____ in double muscle animals?
Definition
decreased due to decreased fibroblasts
Term
What allows tropocollagen to be more tightly packed?
Definition
glycine, allows to form triple helix
Term
How does hydroxyproline aid in collagen synthesis
Definition
only found in CT tissue and used to determine how much CT is in meat
Term
What is tropocollagen synthesized by?
Definition
protofibrils
Term
Once synthesized by protofibrils what is the fate of tropocollagen?
Definition
aligned into a 1/4 stagger leading to unique striations, then assembled into fibrils which are bound to fibers
Term
How many forms of collagen are found in the muscle?
Definition
5
Term
What colour is an individual collagen fiber? a sheath?
Definition
colourless, a sheath is white
Term
What colour is elastin?
Definition
yellow
Term
# of fibrils in a fiber is dependent on?
Definition
load and stress factors for the collagne fiber
Term
How do intramolecular crosslinks affect collagen strength?
Definition
intramolecular crosslinks between PP strands whithin the tropocollagen muscle giving extra tensile strength(intermolecular does this as well)
Term
As intramolecular crosslink diameter _____ you _________ toughness
Definition
increase, increase
Term
as you _____ interfibril noncovalent cross links with small diameter fibers you __________ toughness
Definition
increase decrease
Term
Age effect on collagen
Definition
in young animals rapid growth is seen therefore there are more soluble crosslinks then there are stable crosslinks
Term
What type of cross links are heatliable?
Definition
reducible/soluble crosslinks
Term
What are reducible crosslinks converted to in older animals?
Definition
pyridinoline, which forms non-reducable cross links which increase overall strenght-low solubility
Term
How is age assessment done?
Definition
by looking at how much cartilage has been changed to bone
Term
can diet effect crosslink stability?
Definition
yes
Term
How can you increase collagen turnover(via diet) in cattle?
Definition
by feeding high energy diets
Term
True or False
Feeding high grain diets to cattle increases collagen turnover
Definition
true
Term
True or False
there is more hydroxyl proline in pork than beef
Definition
true
Term
True or False
Toughness is correlated with collagen in peri, epi and endomysium
Definition
False, just with peri and endo
Term
Elastin is found in
Definition
Arteries-to maintain structural integrity with changes in blood flow
Abdominal wall
Ligamenta nuchae
Lungs
Skin
Small amounts in meat-varies depending on muscle function
Term
Elastin is resistant to?
Definition
acids
bases
heat
digestive enzymes
Term
Elastin is made up of which amino acids?
Definition
glycine
desmosine
hydroxyproline
Term
What aa is key in elastin formation?
Definition
desmosine
Term
How is desmosine formed?
Definition
by lysine which by the enzyme lyslyl oxidase and copper(co factor) is turned to desmosine
Term
How does copper deficiency lead to aortic rupture?
Definition
if copper is deficient desmosine cannot be formed and this affects structure of the aorta which can cause aortic rupture
Term
Where is the strongest cnnective tissue found?
Definition
the forelimb and the hindlimb
Term
cooking tough meat converts CT to?
Definition
gelatin
Term
smaller sarcomere leasds to ___ crosslinking which leads to ____ beef
Definition
more , tougher
Term
How does cooking with moisture(water) affect collagen?
Definition
hydrates and hydrolyses(breaks down) collagen changing it's solubility and converting it to gelatin
Term
True or False
Type II collagen is more heat susceptible?
Definition
False
Type I collagen is more heat susceptible
Term
What is papain?
Definition
enzyme used in cooking to denature proteins
Term
True or False
Adipose is a connective tissue
Definition
True
Term
Marbling fat accounts for what % of tenderness?
Definition
10%
Term
When adipose tissue is beneath the skin it is?
Definition
subcutaneous
Term
When adipose tissue is found between the muscle it is?
Definition
intermuscular
Term
Within the muscle adipose tissue is known as?
Definition
intramuscular
Term
Where does adipose tissue originate from?
Definition
Mesoderm-which is one of the 3 primary germ lines that produce all tissue for the body
Term
What are the 3 primary germ lines that produce all the tissues in the body?
Definition
ectoderm, endoderm and mesoderm
Term
What hormones differnetiate adipose from other connective tissue?
Definition
insulin, thyroid hormone and IGF-1
Term
Once a cell starts to store ____ it can no longer divide
Definition
triglycerides
Term
Adipocytes mainly filled with?
Definition
lipid
Term
What affect do the lipid filled adipocyte have on the cellular organelles?
Definition
the nucleus, mitochondria, organelles and cytoplasm are all pushed to one side which results in low water content
Term
What is an adipose tissue depot?
Definition
an accumulation of adipocytes
Term
Do adipose have well developed capillaries ?
Definition
yes for depositing into fat depots and for mobilizing when fat is needed
Term
What is the lace fat?
Definition
CT supporting the intestines
Term
What is omentum?
Definition
the thin connective tissue sack filled with fat that covers the stomach and intestines
Term
What are the phases of fat deposition?
Definition
internal fat
subcutaneous and intermuscular
intramuscular-can be mobilized
Term
Who is leaner gilts or barrows
Definition
gilts are leaner than barrows
Term
what is cellular hyperplasia?
Definition
increased cell numbers
Term
what is cellular hypertrophy?
Definition
increased cell size
Term
What is marbling associated with?
Definition
increased tenderness
Term
How does IMF help in mastication?
Definition
acts as a lubricant
Term
Juiciness is attributed to?
Definition
IMF and water in meat
Term
What impacts meat flavour?
Definition
carbonyl compounds in adipose tissue and presence of specific fatty acids
Term
Brown fat is found in?
Definition
new borns, cold adapted animals and hibernating mammals
Term
What is brown fat used for?
Definition
temperature regulation as it acts as a site for heat production
Term
After chronic exposure to cold heat is produced without shivering how?
Definition
thyroxine-increases BMR
Catecholamines-increase metabolism in all fats, especially brown
Term
True or False
Brown fat produces heat and energy?
Definition
false
it does NOT produce energy
Term
What is lipogenesis?
Definition
fat synthesis
Term
what is lipolysis?
Definition
fat breakdown
Term
What is ketosis referred to as in ewes?
Definition
pregnancy toxemia
Term
What is the makeup of a triglyceride?
Definition
3 fatty acids bound to a glycerol backbone
Term
What is the makeup of a triglyceride?
Definition
3 fatty acids bound to a glycerol backbone
Term
Where are triglycerides hydrolyzed?
Definition
pancreatic lipase hydrolyzes in the small intestine
Term
What happens when the products of pancreatic lipase hydrolysis are absorbed?
Definition
they are resynthesized into a triglyceride, and is then packed with a protein and cholesterol to form chylomicron which are picked up by the lymphatic system and transported to the adipose tissue
Term
Are tags absorbed by the adipose tissue
Definition
no
Term
What happens when tags reach the adipose tissue?
Definition
hydrolyzed into free fatty acids and glycerol where the FFA enther the adipose and the glycerol enters the blood stream for metabolism
Term
What happens to the melting point of fat if fed UFSA?
Definition
the fat will have a lower melting point which is associated with runny pork which leads to decreased shelf life and odors
Term
What happens when ruminants consume UFSA?
Definition
they will be predominantly hydrogenated in the rumen resulting in mostly SFA depositing in the tissue
Term
Which produces higher UFSA concentration in ruminant tissue?
Definition
forages do compared to grain
Term
True or False
Monogastrics can synthesize CLA
Definition
False, ruminants can. To increase CLA in monogastric meat they must be fed it
Term
How can you increase fat content in ruminant diets
Definition
You cover the fat with formaldehyde to prevent micro-organisms from attacking them allowing them to be digested by the animal in the intestines
Term
Is glucose a substrate in lipogenesis in ruminants
Definition
No, as they cannot absorb glucose well in the intestine, they use VFA instead
Term
Where does lipogensis occur in chickens?
Definition
the liver and not the adipose tissue
Term
Implants effect:
Definition
increase gain and feed efficiency
increase muscling and retail yield
decrease all fat deposition
Term
What type of implants are there?
Definition
estrogen based
testosterone
combination
Term
What is organic containing matter?
Definition
carbon containing material that is mainly proteins
Term
What is inorganic matter?
Definition
minerals
Term
Organic part of bone provides?
Definition
Resilience, toughness and flexibility
Term
___% of the bone tissue is inorganic salts
Definition
67
Term
Inorganic part of bone provides?
Definition
hardness, rigidity and resistance to x-rays
Term
What does decalcification do to the bones?
Definition
by treating with acid you eliminate the rigidity of bone so it becomes flexible
Term
Functions of bone?
Definition
protection, a lever for locomotion and mineral storage
Term
What are canaliculi?
Definition
regions where diffusion takes place and allow cell to recieve nutrients and dispose of waste
Term
What is the cancellous bone?
Definition
central portion filled with bone spicules or trabeculae
Term
What are spicules?
Definition
have alot of pace between them which are occupied by bone marrow and blood vessels which pulls bone as muscle contracts and acts as a shock absorber
Term
Where is cancellous bone found?
Definition
in the extremities of long bones
Term
What is unique about red marrow?
Definition
found in young animals but converted to yellow with age except in cancellous bone
Term
What are compact bones?
Definition
heavy, dense and strong and found in shafts of long bone and the outside layer of all bones
Term
True or False
Haversian canals contain blood vessels
Definition
true
Term
What are the components of long bones?
Definition
epiphysis
diaphysis
epiphyseal plate
articular cartilage
endosteum
periostuem
Term
What is the Epiphysis
Definition
make up the extremitie of the bone
made of both types of bone
provide lightness and strength
shock absorber
Term
What is the Diaphysis?
Definition
also called the shaft
lies between extremeties
contains marrow or medullary cavity
covered by compact bone
where bone increases during growth
Term
What is the Epiphyseal plate(physis)
Definition
the growth plate
layer of hyaline cartilage between ends and shaft of long bone-->eventually converted to bone
where bone will increase in lenght
Term
what is articular cartilage
Definition
on the surface of compact bone where one bone will come in contact with another bone to form a joint
Term
what is the endostuem?
Definition
lines all surfaces facing medullary cavity and contains osteoblasts and osteocrasts
Term
what is the Periosteum?
Definition
covers all bones except for joint surfaces where articular cartilage is present
Term
What part of the bone increases shaft diameter and heals fractures?
Definition
the periosteum
Term
What type of marrow aids in blood formation?
Definition
red
Term
What type of bone marrow stores fat ?
Definition
yellow
Term
What is the function of osteoblasts?
Definition
synthesizes bone
produces bone matrix
Term
What are osteocytes?
Definition
mature bone cell
maintains bone matrix
can become osteoblasts
Term
what are osteoclasts
Definition
large and motile
break down bone
Term
Type of bones?
Definition
Long
Short
flat
sesamoid
irregular
Term
Where is cartilage key?
Definition
in the respiratory tract for the larynx, trachea and bronchi
Term
3 types of cartilage?
Definition
hyaline
elastic
fibro
Term
Where is hyaline cartilage found?
Definition
in joint surface to provide smooth surface
reduces friction and wear/tear for bones
Term
where are elastic cartilage found?
Definition
inner ear, epiglottis and larynx
Term
where are fibro cartilage found?
Definition
inter-vertebral disks of spine, pelvic bones and knee joints
Term
Difference between bone and cartilage?
Definition
cartilage is softer, more flexible/resilient, can't regenerate as easily as bone can
Term
What can bone formation also be referred to as?
Definition
ossification or osteogenesis
Term
3 types of bone formation?
Definition
heteroplastic
endochondral
intramembranosus
Term
Where does heteroplatic bone formation occur?
Definition
non-skeletal sites
Term
What type of bone formation synthesizes membrane bone?
Definition
intramembranosus
Term
Intramembranosus bone formation is used in the formation of?
Definition
flat bones of the skull, face, mandible,clavicle
Term
Which farm species contains a clavicle
Definition
bird BUT NOT PIGS OR BEEF
Term
Bone is formed on the _____ and removed from the _________
Definition
outside, inside
Term
What happens to bone mass when muscle mass decreases?
Definition
it decreases as well
Term
Process of bone formation:
Definition
shrink(atrophy)
increase in size(hypertrophy)
repair breaks
rearrange internal structure to deal with stresses and strain as bone subjected to movement
Term
What hormone is responsible for increase release of Ca salts from the bones? how does it do this?
Definition
Parathyroid glad senses low Ca, PTH increases release of Ca from bones by increasing osteoclasts, decreasing ostebolasts and increasing osteocytes
Term
How does calcitonin prevent hypercalcemia?
Definition
deposits excess Ca into bones
Term
How to cells specialize into osteoblasts or cartilage?
Definition
no capillaries become cartilage
capillaries become osteoblasts
Term
What happens to the right ovary in avians?
Definition
lost at day 10 of incubation
Term
when does the oviduct start to develop?
Definition
15 to 16 WOA
Term
How is the ovary activated?
Definition
by light stimulating the anterior pituitary to secrete FSH
Term
11 days before first egg is layed what occurs?
Definition
FSH released which causes ovarian follicles to grow in size, hormone synthesis occurs(estrogen, progesterone and testosterone), development of medullary bone for Ca supply for egg shell,
Term
where are albumen proteins, shell membranes and the cuticle synthesized?
Definition
the oviduct
Term
how long does the yolk take to mature?
Definition
10 days
Term
Release of mature ovum is due to?
Definition
progesterone production in the ovary
LH hormone
ovulation of mature ovum
released into body cavity
Term
The ovum is caught by?
Definition
the infundibulum
Term
Components of the avian oviduct include?
Definition
infundibulum
magnum
isthmus
shell gland/uterus
vagina
Term
What is the purpose of the infundibulum?
Definition
to search for and engulf the yolk, it stays here for 15-18min
Term
Where is the first layer of albumin formed?
Definition
in the infundibulum, yolk is fertilized first
Term
where is most of the albumen secreted?
Definition
magnum
Term
what does the albumen function to do?
Definition
prevent invasion and growth of microbes to protect the embryo
contains protesase inhibitors and chelators
source of water, protein and minerals BUT NOT FAT
Term
where are the inner and outer shell membrane formed?
Definition
isthmus
Term
what is the name of the last albumen layer and where is it synthesized?
Definition
outer thin white
shell gland
Term
where does calcification occur?
Definition
shell gland
Term
where does Ca in the shell come from?
Definition
diet and medullary stores
Term
how is the vagina separated from the shell gland?
Definition
uterovaginal sphincter
Term
when is the cuticle formed?
Definition
30 mins before oviposition
Term
clutch is when?
Definition
# of consecutive days when eggs are being laid
Term
what is a pause?
Definition
time between clutches when eggs aren't laid
Term
longer the clutch the ____ the pause
Definition
shorter
Term
when is sexual maturity in avains?
Definition
17-19wks
Term
What is egg production like for the first 2 weeks?
Definition
erratic, having double yolks and poor quality shells
Term
around what age does egg production decline?
Definition
40-50 weeks
Term
Male repro tract:
Definition
1. testis
2. Epididymis
3. Vas deferens
4. Papilla
5.Urodaeum or cloaca
6. Ureter
Term
What sex glands do birds lack that mammals possess?
Definition
seminal vesicles
prostate
bubourethral glands
Term
How are birds nasal and oral passages separated?
Definition
via slit in roof of mouth
Term
Do birds have a distinction between mouth and pharynx like other animals?
Definition
nope
Term
True or False
Birds have larger number of taste buds
Definition
true
Term
What are the 3 regions of the cloaca?
Definition
digestive
urinary
repro
Term
Does the size of a birds "vent" change with age?
Definition
yes as well as gender
Term
Why do birds excrete uric acid?
Definition
less water loss than urea and is less toxic to the developing egg
Term
compare mammalian trachea to avian?
Definition
mammalian C shaped
avian has complete rings
Term
What muscle drives the value of the bird vs red meat?
Definition
bird-breast
meat-longissimus dorsi
Term
How has the vertebral column adapted for flight in birds?
Definition
shortened fused vertebral column in trunk, don't see this in mammals
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