Term
| What is the smallest unit we can record? |
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Definition
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Term
| What do current and voltage clamps measure? |
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Definition
- Current clamp: intracellular, population, currents in the cell, the neurons themselves
- Voltage clamp: whole cell patch, all receptors of one cell |
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Term
| What is membrane potential? |
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Definition
In living cells there is a potential difference between the inside and outside of the cell.
When a channel opens, this difference makes ions flow. This generates current, which we can record. |
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Term
[image]
- What kind of patch clamp recording is this?
- What does 0pA and 2pA refer to? |
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Definition
- Singe channel recording / voltage clamp
- 0pA = channel is closed, 2pA = channel is open |
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Term
[image]
- What kind of patch recording is this?
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Definition
| - Whole-cell / voltage clamp |
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Term
[image]
- What kind of patch recording is this?
- What are the spikes? |
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Definition
- Whole-cell / current clamp
- We're clamping the current, which allows us to measure the voltage (the pressure that pushes the current) |
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Term
| What sorts of questions can whole cell recordings answer? |
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Definition
- What are the characteristics of individual cells?
- What are the receptor population and dynamics like? |
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