Term
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Definition
Hydrolytic enzyme (produced in fruit) active during the ripening process (to help soften fruit)
presence of activity -- determined by clearing around the bacteria where cellulase has broken down the cellulose
measured -- gel diffusion (hydrolysis of carboxymethylcellulose (CMC) |
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Term
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Definition
usage of starch-- organism must produce enzyme that breaks molecule's two components (amylose (unbranched glucose polymer, amylopectic (larch branched polymer) into smaller sugar units
2 bacteria will be compared for ability to breakdown the carbohydrate starch |
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Term
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Definition
enzyme -- hydrolase that the organism secretes and is involved in the reaction"
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starch+H20 -> dextrins+maltose+glucose
amylase will diffuse through the agar and digest the starch (Gram's iodine, detects presence of this enzyme) Areas where starch been broken down (will be clear-- indicating produced a-amylase) |
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Term
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Definition
| soluble mixture of peptides made by boiling tissue collagen. |
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Term
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Definition
Bacteria have the ability to break down gelatin by producing enzyme (gelatinase)
presence of gelatinase: pathogenicity of organism (ability to breakdown connective tissue allowing organsim to penetrate deeper into tisse and spread throughout host)
37 C - liquefy
4C resolidify
If hydrolized will remain liquid which indicates (positive gelatinase reaction) |
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Term
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Definition
converts hydrogen peroxide to water and oxygen
test: addition of H2O2 (2's are at bottom like a base) to the culture
2H202 --> (catalase is on bottom)
bubbles will be released ( indicating a positive reaction) |
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Term
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Definition
during aerobic respiration electrons are passed along the electron transport chain -- resulting in the oxidation and simultaneous reduction of cytochromes and other carriers.
Interference-- death of cell (can no longer generate ATP) by this process.
pink oxidase reagent (tetramethy-p-phenylenediamine dihydrochloride) acts as a substrate that donates electrons to the cytochrome oxidase (proceess being oxidized)
Dark purple -- oxidase positive organisms |
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Term
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Definition
Good for differentiating gram (-) rods
enzyme involved in the oxidation of cytochrome c (italic c) during this process using O2 (2 is at the bottom) as an electron acceptor resulting in the formating of H20.
Strict aerobic bacteria-- require this enzyme
Facultative anaerobic bacteria-- do not have this enzyme. |
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