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Chromatin that is condensed and not available for transcription, locked files |
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Chromatin that is NOT condensed, DNA IS available for transcription. |
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DNA + Histones ( 4 types) is the bead on the string |
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Histone H1, aids the 10-nm fiber to coil forming the 30-nm chromatin fiber |
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30-nm fiber forms loops that are attached to a chromosome scaffold made of non histone proteins, which makes the 300-nm fiber |
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DNA sequence that regulates binding of transcription factors to promoter |
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Protein that binds to enhancer and stimulates transcription |
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Protein that prevents transcription |
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Transcriptional Gene Silencing |
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Definition
Histone modifications create an environment of heterochromatin around a gene making it inaccessible to Transc. Machinery |
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Translation Gene Silencing |
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Definition
A genes mRNA is destroyed which prevents translation from making a active gene |
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Gene silencing importance |
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It is important because it is used to protect the organisms genome against infectious DNA elements. |
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a post-translation protein that labels proteins for degradation |
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is responsible for the first level of DNA packing in chromatin due to their positively charged amino acids which bind tightly to negatively charged DNA |
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essential for long term inactivation of certain genes, if maintained accounts for genomic imprinting |
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acetyl groups bind to positively charged lysines in histone tails which neutralizes their chrages and they will no longer bind to neighboring nucleosomes |
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Polymerase chain rxn (PCR) |
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Definition
Is used to amplify certain regions of a DNA strand |
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Definition
A DNA polymerase enzyme that transcribes single stranded RNA into single stranded DNA, the reverse of regular transcription |
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Definition
Joins backbones of adjacent Okazaki fragments, forming a single new DNA strand. |
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Is used to check for the presence of a DNA sequence in a DNA sample |
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seperates and unwinds 2 DNA strands |
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Releives strain caused as DNA strands unwind |
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Definition
Adds nucleotides one at a time to 3' end |
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Definition
3' end towards replication fork, easy straight shot replication |
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5' end towards replication fork, elongates in Okasaki fragments. |
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Definition
begins replication by attatching short strand of RNA to template strand |
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Attatches DNA nucleotides to primer |
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Definition
Removes primer and replaces it with DNA nucleotides |
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DNA sequence that marks begining of transcription |
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Definition
Enzyme that separates DNA strand and synthesises RNA strand (5'-3') |
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Specific region of promoter |
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proteins that bind to TATA box, let you know what genes need to be transcribed |
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DNA sequence that signals RNA to be released and transcription sequence to detach |
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Definition
modified guanine added to 5' end of mRNA |
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50-250 adenines added to 3' end |
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Definition
Non-coding regions that are cut out of mRNA before it leaves the nucleus |
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Definition
coding regions that are spliced together after introns are removed |
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Definition
Site of protein synthesis |
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Definition
Hydrolysis of macromolecules occurs |
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Definition
Cellular storage compartments |
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Definition
Synthesis of plasma components occurs |
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Females chose males with certain phenotype |
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Conditions that favor non-average phenotypes |
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Different habitats btw. different species |
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Breeding occurs at different times of the year |
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Courtship rituals differ. |
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Morphological differences prevent mating |
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Definition
Various mechanisma prevent fusion between sperm and egg |
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Term
Oxidative Phosphorylation |
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Definition
The difference in H+ concentrations on opposite sides of the inner mtochondrial membrane is the direct energy source that drives ATP synthesis |
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Term
What is the relationship between the light reactions and the Calvin Cycle |
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Definition
The light reactions provide ATP and NADPH to the Calvin cycle, and theCalvin cycle returns ADP, Pi, and NADP+ to the light reactions |
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Term
What is the path of electron flow during cellular respiration? |
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Definition
Glucose-NADH-electron transport chain-oxygen |
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Where does the Calvin cycle take place? |
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Definition
In the Stroma of the chloroplast |
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Definition
Itroduces a stop codon into the mRNA |
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What does cyclic electron flow in the chloroplast produce? |
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Definition
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Covalent Bonds vs Ionic Bonds |
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Definition
Cov. bnds invovle the sharing of electrons between atoms, and ionic bonds involve the electrical attraction btw atoms |
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