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Manual Cell Counts
68K 12-01 Hemo 101 Unit 3
6
Other
Undergraduate 2
01/13/2012

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Term
Leukocytosis
Definition
Above normal WBC count
Term
Leukopenia
Definition
Below normal WBC count
Term
Condtions that cause Leukocytosis
Definition
Bacterial Infections, Appendicitis, Strep Throat, Heart Attack, certain leukemia, pregnancy.
Term
Leukopenia
Definition
Radiation therapy, chemotherapy, viral states, influenza, antibiotic therapy, certain anemia.
Term
Specimen requirements for Leukocyte count
Definition
EDTA whole blood. WBC Unopenette requires 20 uL.
Term
Principal of Manual Leukocyte Count
Definition
20Ul is added to WBC unopette resorvior, which contains pre-measured valume of diluen (1.98 mL) compoused of ammonium oxalate, Sorensen's phosphate buffer, thimerosal and water. Lyses RBCS preserves WBCs and PLTs. Dilutes dpecimen is added to a hemacytometer for afor a manual count of leukocytes and/or platelets. Stable for 3 hours.
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