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| What are some characteristics of yeast? |
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Definition
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| When are wet mounts used? |
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Definition
-during the hay infusion
-protists will be near top
-the level of light must be reduced to achieve contrast |
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Term
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Definition
| occurs when a microorganism moves by appendages called flagella |
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Definition
| is a shaking type of motion caused by molecules in the liquid striking the organisms causing them to shake or bounce |
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Term
| Characteristics of Protists |
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Definition
-single celled eukaryotic
-has nucleus
-include the animal like group "protozoa"
-protozoa cause malaria, amoebic dysentery, african sleeping sickness, and giardiasis
-bigger than bacteria |
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Term
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Definition
-form more readily on rough and hydrophobic surfaces
-bacteria with flagella attach more easily
-produce alginate
-up to 1000 times more resistant to antimicrobial agents
-65% of all human infections come from biofilms |
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Definition
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Definition
| collection of hyphae (fungal filaments) |
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Definition
| walls seperating hyphae (fungal filaments) |
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Definition
| spores on the end of hyphae |
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Definition
| substances used to kill or inhibit the growth of microbes |
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Term
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Definition
| chemical used on nonliving surfaces |
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Definition
| chemical used on living tissue |
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Term
| What is the difference between bactericidal and bacteriostatic? |
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Definition
bactericidal- an antimicrobial agent that kills bacteria
bacteriostatic- an antimicrobial agent that inhibits growth of bacteria |
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Term
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Definition
| an antimicrobial agent that is dissolved in alcohol (ex. iodine) |
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Term
| Phenol is an effective antimicrobial agent, but why has it not been used for many years? |
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Definition
| it has a harsh odor, and irritates the skin |
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Term
| What is a way to make 100% alcohol even more effective? |
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Definition
| add water because it is needed for hydrolysis |
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Term
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Definition
-selective for staph
-if mannitol is fermented, medium turns from pink to yellow |
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Term
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Definition
| distinguishes between staph (+) and strep (-) |
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Term
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Definition
-staph aureus is distinguished from other staphylcocci by this test
-staph aureus produces coagulase which coagulates plasma to form fibrin |
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Term
| What is the novobiocin test used for? |
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Definition
| to distinguish staph saprophyticus (resistant) from staph epidermis (sensitive) |
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Term
| Commercially available kits for the identificaqtion of staph aureus detect what two things? |
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Definition
| it tests for the presence of coagulase and protein-A (found on the surface of staph aureus [protects from phagocytosis]) |
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Definition
| supports growth of Neisseria and Haemophilus |
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Definition
| both selective and differential for Corynebacterium |
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Definition
| destroys red blood cells and converts hemoglobin to a colorless form |
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Definition
| green discoloration, red blood cells remain intact |
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Definition
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Definition
| using antibodies to identify an unknown organism |
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Term
| Why is it important to poke holes in the blood agar when culturing strep pyogenes? |
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Definition
| to not miss weak hemolysis, the reduced oxygen tension beneath the surface |
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Term
| Where is the antibody/antigen in Rapid Strep A test? |
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Definition
antibody=in kit
antigen=in body |
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Definition
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Definition
| used to distinguish group D strep from other strep |
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Definition
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Term
| Where are the antibody/antigen in a mono kit? |
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Definition
antibody=body
antigen=kit
*healthy people produce forssman antibodies which may lead to a false positive |
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