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Micro-exam 2
microbiology
20
Biology
Undergraduate 4
02/05/2009

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Term
Pseudomonas fluorescens
Definition
Aerobic, gram-,Present in soil, water, plant,Pigments fluoresce
surfaces
Term
Bacteroides
Definition
Strict Anaerobe, gram-, dominant microbe in human feces
Term
Campylobacter jejuni
Definition
gram-, most prevalent food borne illness
Term
Shewanella
Definition
Gram -, non-fermentative, can generate electricity by
respiring on electrode
surfaces.
Term
Anaerobic Respiration
Definition
Uses inorganic and organic molecules other than
oxygen as terminal electron acceptors; this produces
additional ATP for the cell, but not as much as is in
aerobic respiration (due to diff in potential)
Term
Why is there so much
diversity in electron acceptors?
How can prokaryotes
accomplish this?
Definition
Through branched electron transport
chains and modules of oxido-reductases
Term
Nitrate reducing bacteria
Definition
Beneficial process for sewage treatment plants,Dissimilatory nitrate reduction widespread in microbes
– Used for making energy via oxidative phosphorylation
Term
Dissimulative NO3
- reduction
Definition
NO3-->NO2-->NO-->N20-->N2
Term
Four terminal reductases
Definition
NAP, NOR, NIR, N2OR
Term
What nitrogen compounds can be reduced by a nir mutant?
Definition
Term
Periplasmic nitrate reductases (NapA)
Definition
contains
a molybdenum cofactor
Term
ATP synthesis and Dissimilatory nitrate reduction
Definition
ATP synthesized by oxidative phosphorylation
Term
Where are enzymes located in dissimilatory nitrate reduction?
Definition
In the membrane
Term
Nernst Equation
Definition
(Delta)G=-nF(delta)E
Term
Arsenic poisoning
Definition
Microbes respire arsenate, made arsenite. Causes DNA damage, affects ATP synthesis
Term
Where does the remaining energy from making ATP go?
Definition
Term
Sulfate reducing bacteria
Definition
Deltaproteobacteria, anaerobes, use H2, lactate/acetate/ethanol for carbon/energy source. Common in sulfur rich environments (seawater)or places w/ decomposing matter.
Term
Chemolithotrophy/Oxidation of inorg molecules
Definition
A. A pathway used by a small number of microorganisms
called chemolithotrophs
B. Produces a significant but low yield of ATP
C. The electron acceptor is commonly O2, some others
include sulfate and nitrate
D. The most common electron donors are hydrogen,
reduced nitrogen compounds, reduced sulfur
compounds, and ferrous iron (Fe2+)
Term
Typical habitats
of chemolithotrophs:
Definition
Near the interface of
oxic/anoxic conditions
Term
Oxidation of sulfur-compounds
Definition
Produces sulfuric acid (H2SO4)
– Acidification of soil
– Dissolution of minerals, e.g. CaCO3
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