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Microbiology Lab
Exercise 3
21
Biology
Undergraduate 3
01/16/2008

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Term
List the five characteristics, of the organisms that cause microbial infections, that provide a profile which is used by the medical microbiologist for preliminary identification?
Definition
Size
Shape (rods or spheres)
Gram Staining Reaction of individual cells
Growth requirements
Colony morphology
Term
Define pyogenic.
Definition
pus-forming
Term
What are the three major groups that make up the "Pyogenic Cocci"?
Definition
Staphylococci
Streptococci
Neisseria
Term
Which major group are Gram + cocci which form grape-like clusters?
Definition
Staphylococci
Term
Why do the staphylococci form grape-like clusters?
Definition
Their plane of cell division is random and they do not separate.
Term
Which group of organisms form opaque colonies that have a glossy appearance, are yellow to white in color, and are usually facultatively anaerobic?
Definition
Staphylococci
Term
Which bacteria divide in a single perpendicular plane and form short or long chains?
Definition
Streptococci
Term
Which group of microbes are Gram +, grow well on rich media, and are either aerotolerant or anaerobic?
Definition
Streptococci
Term
Which group of microbes are the most common Gram - cocci of medical importance?
Definition
Neisseria
Term
Where does Moraxella catarrhalis inhabit? What does this strain frequently cause?
Definition
Inhabits the mucosa.
Causative agent of a lower respiratory disease.
Term
Strains of ___________ can cause typhoid fever and gastroenteritis and are among the most significant pathogens in non-purified drinking water?
Definition
Salmonella
Term
Salmonella are related to __________ which are normally found in the large intestine.
Definition
E. coli
Term
Yeast appear Gram + or Gram - on staining?
Definition
Gram +
Term
What distinguishes yeast?
Definition
Large size and oval shape
Term
Give one example of a yeast species that can reside without harming and inside the human body but can eventually cause disease in individuals with lower defenses or those individuals on some kind of antibiotic? What is the general name given to these yeast species?
Definition
Candida albicans
Opportunist pathogens
Term
List the two bacteria mentioned in the lab that are present in dental plaque.

How do the metabolic activities of these two bacteria contribute to the formation of dental caries?
Definition
Streptococcus mutans and Streptococcus gordonii.
Lactic acid production.
Term
List some infections that Candida albicans cause.
Definition
Oral and vaginal "thrush"
Skin infections
Endocarditis
Oropharyngeal candidiasis
Term
Bacillus anthracis is primarily a pathogen of _______ but is responsible for _________ affecting humans. Clostridium tetani causes ________________.
Definition
Livestock
Anthrax
Wound Infections
Term
What color is usually an indicator of a gram negative bacteria?
Definition
Reddish-pink
Term
What color is usually an indicator of a gram positive bacteria?
Definition
Purple
Term
Which type of bacteria has a think peptidoglycan layer? (Gram + or gram -)
Definition
Gram +
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