Ph

Temp

Oxygen

Nutrients

Coccus - round

Bacillus - rod

Spiral

Pleomorphic - a shape that ranges from rod to coccus

Singel - cocci

Pairs - diplococci

Chains - strep - chain

Clusters - staph

Stain purple

Thick, uniform cell wall

Stain pink or red

More complex structure

Less common

resistant bodies formed by some bacteria

Malachite green stain

Resistant to heat, chemicals, and radiation

Pressure and moist heat kill them

Autoclave - 121 degrees C 15 mins

Lag phase

Exponential growth phase

Stationary phase

Logarithmic decline stage

Take 30 mins - 20 + hours depending on type of bacteria

Single strand DNA

Binary fission

All have potential to be pathogenic

PPE

Dissinfect - let cleaner sit for 20 mins

Swabs

Media plate

Inocculation loops

Solid or liquid

Supports bacterial growth

Powder or preformed

Agar - seaweed/gelaten

Enriched media

Chocolate agar - heat treated blood

Alpha hemolysis - greenish and slimy appearance

Beta hemolysis - turns clear

Gamma hemolysis - no change

Delta hemolysis - doulbe hemolysis, thick border

Selective differential

Contains crystal violet, bile salts, and indicators

Growth/no growth

Liquid medium

Can differentiate between obligate aerobes and obligate anerobes

Aerobes at top

Anerobes at bottom

Falculative at middle - spread out anerobes

ID salmonella and enteric bacteria

Red to yellow

General purpose

Antibiotic sensitivity test

Used to cultivate fungi

Inhibits growth of most bacteria

Takes about a month to complete

Aspirate

Swab

Skin scrap

Pluck hair