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Microbiology
Final Exam
220
Other
Undergraduate 3
12/09/2006

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Term
Purple Bacteria
Definition
All light reaction business within cytoplasmic membrane.
Reverse electron transport part of lifestyle for this phototroph.
Term
Green Bacteria
Definition
Chlorosomes contain antennae pigments
Term
Acidithiobaccilus ferroxidans
Definition
Fe+2//O2
Rusticyanin involved in oxidation.
Term
Nitrosomonas
Definition
NH3//O2 > NO2-1
Term
Nitrobacter
Definition
Oxidizes nitrite for a living
Term
Methanogens
Definition
These guys provide "natural gas".
Term
sulfidogens
Definition
This poor neutraphile croaked on it's acid.
x//SO4-2.
Term
Ralstonia
Definition
H2//O2.
Two hydrogenases produce reducing equivalents and ATP.
Term
Cyanobacteria
Definition
Water electron donor for these guys.
Term
Yes
Definition
Does respiration require a membrane?
Term
Yes
Definition
Does respiration require ATPase?
Term
Yes
Definition
Does respiration require an electron donor?
Term
Yes
Definition
Does respiration require an electron transport system?
Term
No
Definition
Does respiration require the Embden Meyerhof Parnas Pathway?
Term
No
Definition
Does respiration require the reduction of pyruvate?
Term
No
Definition
Does respiration require substrate level phosphorylation?
Term
No
Definition
Does respiration require the TCA cycle?
Term
Yes
Definition
Does respiration require a terminal electron acceptor?
Term
gas vesicles
Definition
aquatic phototroph depth finder
Term
pili
Definition
bacteria conjugation
Term
periplasm
Definition
between the membranes in Gram negative bacteria
Term
magnetosomes
Definition
chemoorganotrophic bacteria locate depth in the pond
Term
porin
Definition
forms holes in outermembrane
Term
lipoteichoic acid
Definition
holds wall to membrane (Gram positive)
Term
teichoic acid
Definition
like rebar in Gram positive walls
Term
endospores
Definition
peculiar to Bacillus and Clostridium
Term
typeIV fimbriae
Definition
specific attachment like Neisseria and Vibrio - mostly protein
Term
lipid a
Definition
toxic moiety of outer membrane
Term
Needham
Definition
cork sealed flasks
Term
Winogradsky
Definition
discovered lithotrophs
Term
Leeuwenhoek
Definition
elected to the Royal Society for discovery of microbes
Term
Redi
Definition
flies have mommies and daddies
Term
Spallanzani
Definition
hermetically sealed flasks
Term
Count Buffon
Definition
philosopher - in league-colleague with Needham - "heat of course, destroys the vital force"
Term
Pasteur
Definition
swan-necked flasks
Term
Winogradsky
Definition
tried in his own pitiful way to grow Beggiotoa in flasks
Term
prion
Definition
"replicative" protein infectious element.
BSE.
Kuru.
Term
charon
Definition
Carries the sould over the river Styx (not new testament doctrine)
Term
rota
Definition
Children's diarrhea
Term
pox
Definition
Class 1, replicates in cytoplasm
Term
parvo
Definition
Class 2, animal host
Term
X174
Definition
Class 2 phage
Term
rhabdo
Definition
Class V, replicates in cytoplasm, bullet-shaped
Term
retro
Definition
Class VI.
genome = 2 molecules of RNA derived from dsDNA intermediate.
RNA genome (in virus), DNA genome replicative form.
Term
hepB
Definition
Class VII
Covalent modifications of host RNApol, for early, mid and late proteins.
DNA genome (in virus), RNA genome replicative form
Term
M13
Definition
Cloning vector picture, "clear" and blue plaques
Term
pBR322
Definition
Constructed plasmid cloning vector.
Several restriction sites (poly linker FYI) reside within gene for tetracyline resistance.
Term
polio
Definition
current REQUIRED US vaccine "killed".
giant protein cleaved for active products.
positive strand RNA animal virus.
Term
herpes
Definition
DNA virus, envelope from nucleus
Term
influenza
Definition
drift accounted for in yearly vaccines.
Killed more people in the shortest period of time.
Segmented genome recombination from alternate sources possible.
Term
YAC
Definition
Eukaryote artificial chromosome large scale cloning.
Term
T7
Definition
Its polymerase ONLY recognizes phage promoters
Term
MS2
Definition
Only 4 proteins!
maturation, coat, replicase, lytic
Term
viroid
Definition
Plant diseases, requires insect vector.
Virus without a coat.
Term
Mu
Definition
Rude lysogenic phage inserts DNA into the middle of host genes!
Term
T4
Definition
Special base, 5-hydroxymethylcytosine
Term
lysogeny
Definition
(Lambda) increased concentration cI

lytic or lysogeny?
Term
lytic
Definition
(Lambda) cII degradation
Term
lytic
Definition
>Cro
Term
lysogeny
Definition
(Lambda) integrase
Term
lysogeny
Definition
(Lambda) Leftward transcription
Term
lytic
Definition
(Lambda) Rightward transcription
Term
False
Definition
True or False - In E.Coli K12 there is always one copy per cell
Term
true
Definition
True or False - E.Coli K12 has been completely sequenced
Term
false
Definition
True or False - In E. Coli K12, <1% represents "foreign" DNA
Term
True
Definition
True or False - E.Coli K12 includes plasmids
Term
True
Definition
True or false - E.Coli K12 includes phage
Term
False
Definition
True or False - E.Coli K12 is transcribed entirely clockwise
Term
False
Definition
True or False - In E.Coli K12, 90+% of the gene products are known
Term
positive control - induction
Definition
activator + inducer: complex binds to mRNA
Term
feed-back inhibition
Definition
allosteric anabolic enzyme involved
Term
covalent enzyme modification
Definition
concentration of glutamine regulated this.
example involved CAP and cyclic AMP.
Term
negative control - induction
Definition
corepressor binds to activator: this complex binds to operator
Term
negative control - repression
Definition
corepressor binds to repressor: this complex binds to operator
Term
product inhibition
Definition
function of Keq
Term
isozymes
Definition
different proteins catalyze same reaction, subject to different regulatory controls
Term
alternative sigma factors
Definition
required for promoter recognition - part of RNA polymerase
Term
global control
Definition
SOS response
Term
quorum sensing
Definition
Staphylococcus in boil.
Vibrio fischeri.
Term
size of typical bacterium
Definition
mega basepairs
Term
size of typical archae
Definition
mega base pairs
Term
size of typical herpes virus
Definition
kilo base pairs
Term
size of typical human genome
Definition
giga base pairs
Term
size of typical viroid
Definition
hecto(1000) base pairs
Term
nonsense mutation
Definition
stop codon introduced
Term
Frame-shift mutation
Definition
insertion/deletion
Term
Silent mutation
Definition
all amino acids in the resulting protein, same as wild-type
Term
missense mutation
Definition
it usually will not make a difference in the average protein
Term
transformation
Definition
capsule, pneumonia.
Griffith did this.
naked DNA.
Term
conjugation
Definition
F factor.
Hfr strains.
Pilus.
Term
transduction
Definition
phage involved.
toxin diphtheria
Term
False
Definition
True or False - The walls of archae have peptidoglycan.
Term
True
Definition
True of False - Archae ribosomes = 70S
Term
False
Definition
True or False - Archae lipids are ester linked
Term
True
Definition
True or False - Archae cells are prokaryotes
Term
True
Definition
True or False - Archae have histone-like proteins with DNA
Term
No
Definition
Do proteins with hydrophilic cores contribute to hyperthermophily?
Term
Yes
Definition
Do proteins with more ionic interactions on the protein surfaces contribute to hyperthermophily?
Term
Yes
Definition
Do chaperonins contribute to hyperthermophily?
Term
Yes
Definition
Do histones contribue to hyperthermophily?
Term
No
Definition
Do negative DNA supercoils contribute to hyperthermophily?
Term
False
Definition
T or F - Super hot environments seem like a wonderful place for spontaneous generation (bioneogenesis)
Term
False
Definition
T or F - Similarities in 16s RNA sequences make a lot of practical sense in understanding what bacteria do in the world.
Term
True
Definition
T or F - Short branches in phylogeny trees equal slow evolution
Term
False
Definition
T or F - Ferredoxin is very electropositive
Term
False
Definition
T or F - Parasite usually evolve before their hosts
Term
True
Definition
T or F - According to the Woese scheme, you are more related to a mushroom than a bacterium to an Archae.
Term
True
Definition
T or F - There are more lithotrophs than heterotrophs amongst the hyperthermophiles
Term
False
Definition
T or F - The "sewage fungus" is a eukaryote.
Term
True
Definition
T or F - Protozoa are eukaryotes.
Term
Nanoarchaeum
Definition
"parasite" of Ignicoccus
Term
Bacillus
Definition
1st genetically engineered plants: Frankenfoods, or fewer pesticides from the air?
Term
Methanosarcina
Definition
Acetotrophic methanogen
Term
Clostridium
Definition
Anaerobe, strict fermenter, endospore
Term
Stigmatella
Definition
bacterium, genome twice the size of E.Coli, complex lifestyle
Term
Planctomyces
Definition
Bacterium, stalk, S-layer "covers" cell
Term
Deinococcus
Definition
Can tolerate 1000 times more radiation than you.
Term
Acidianus
Definition
Can use sulfur as either electron donor or acceptor, sounds "two faced"
Term
Cytophaga
Definition
Cellulose, peptidoglycan good groceries, glider
Term
Acetobacter
Definition
CH3CH2OH//O2 > CH3COOH
Term
Zymomonas
Definition
chew and spit corn into a jug. Wait a week. Invite friends for spittle cocktail (Drink responsibly)
Term
Thermomicrobium
Definition
di-alcohol head group, hydrocarbon tail- doesn't look like any other membrane lipid
Term
Chlamydia
Definition
elementary/reticulate bodies, intracellular energy parasite---functional ATPase?
Term
Zymomonas
Definition
Entner Doudoroff - fermenters - sounds stupid to us
Term
Ferroglobus
Definition
Fe+2//NO3-1? Prove it!
Term
Wolbachia
Definition
Feminizing insects - don't ask, dont tell; or a liberal agenda?
Term
Campylobacter
Definition
gastroenteritis, microaerophile!
Term
Corynebacterium
Definition
Greek word for club
Term
Natronobacterium
Definition
halophile, alkalinophile (soda lakes)
Term
Anabaena
Definition
Heterocysts protect nitrogenase.
NO2//O2
Term
Chloroflexus
Definition
hybrid photoheterotroph, chlorosomes
Term
Methanopyrus
Definition
hydrothermal vents, distant relative of other methanogens
Term
Spiroplasma
Definition
insect disease, no cell wall, helical shape
Term
Gallionella
Definition
iron stalk. lithotroph?
Term
Streptomyces
Definition
Lots of antibiotics made by these guys
Term
Listeria
Definition
low morbidity, high mortality, cheese, meat, will grow in the refridgerator
Term
Aquifex
Definition
most thermophilic bacterium, reverse TCA
Term
Propionigenium
Definition
No ETS, Na-ATPase, Na extruding decarboxylase, heterotroph - What's this about Dr. Mitchell?
Term
Thermodesulfobacterium
Definition
3 carbon "back bone" (not glycerol), two hydrocarbons tails, ether linkage
Term
Spaerotilus
Definition
nuisance bug, iron sheaths
Term
Thioplaca
Definition
Ocean floor mats,
H2S//NO3-1
Term
Leptothrix
Definition
precipitates Mn+4
Term
Ectothiorhodospira
Definition
produces S outside cell wall, no chlorosome
Term
Treponema
Definition
Protoplasmic cylinder, syphilus
Term
Chromobacterium
Definition
purple pigment, relative of Neisseria?
Term
Rhodopseudomonas
Definition
Purple non-sulfur bacterium
Term
Sarcina
Definition
pyloric ulcers
Term
Beggiatoa
Definition
removes H2S from rice rhizospheres
Term
Rickettsia
Definition
Rocky Mountain Spotted Fever
Term
Nanoarchaeum
Definition
Smallest genome that we know of - 0.5 megabase pairs
Term
Mycoplasma
Definition
steals sterols from host
Term
Desulfotomaculum
Definition
sulfidogen, spoiler of canned goods
Term
Thermoplasma
Definition
Tetra-ethers, high-temperatures, no wall no problem
Term
Oscillatoria
Definition
this photosystem II containing procaryote, fixes nitrogen by night
Term
Prochloron
Definition
this prokaryote has chlorophyll a and b
Term
Mycobacterium
Definition
walls made of wax, speedy growth lacks!
Term
Verrucomicrobium
Definition
warty little devil - but you know how oligotrophs are
Term
Archaeglobus
Definition
What was this little dude doing in the sulfidogen table in the bacteria chapter?
Term
cell wall
Definition
beta lactam - target
Term
erythromycin
Definition
50s - ribosome
Term
quinolone
Definition
target - DNA gyrase
Term
cephalosporin
Definition
target - cell wall
Term
daptomycin
Definition
target - cell membrane
Term
sulfanilimide
Definition
target - growth factor analog
Term
tetracycline
Definition
target - 30s ribosome
Term
secondary clarifier
Definition
MOST BOD removed
Term
secondary clarifier
Definition
return sludge comes from (as in originates---like the pipe) here
Term
primary clarifier
Definition
primary waste treatment
Term
water hyacinth lagoon (example in class at Epcot center)
Definition
tertiary waste treatment
Term
Zooglea "flocs out" or settles here
Definition
aeration basin
Term
anaerobic sludge digester
Definition
methanogens found here
Term
water hyacinth lagoon (example in class at Epcot center)
Definition
nitrogen and phosphorus removed here
Term
primary clarifier
Definition
bacterial bodies decompose here removing solid waste
Term
anaerobic sludge digester
Definition
floc contacts oxygen and organics oxidizing them to CO2
Term
IgM
Definition
pentavalent, shows up first, low specificity, high avidity
Term
IgD
Definition
don't know much - B lymphocyte surface binding
Term
IgA
Definition
saliva, colostrum
Term
IgE
Definition
allergy, release of histamines
Term
IgG
Definition
high specificity, high affinity major circulating antibody
Term
Mycobacterium
Definition
"colony" in Carville, LA
Term
Salmonella
Definition
big killer in US before filtration and chloronation of water supplies
Term
Vibrio
Definition
death by dehydration
Term
Streptococcus
Definition
Dextransucrase = dextran (plaque) + lactic acid = tooth decay
Term
Borrelia
Definition
disease discovered in Connecticut children
Term
Entamoeba
Definition
eucaryote, dysentery
Term
Streptococcus
Definition
exotoxin A/scarlet fever
Term
Yersinia
Definition
flea bite
Term
Giardia
Definition
foul smelling explosive diarrhea - no blood in stool
Term
Trichomonas
Definition
foul smelling vaginal discharge, males asymptomatic
Term
Cryptosporidium
Definition
gigantic tap water epidemic in Milwaukee
Term
Mycobacterium
Definition
Hansen's disease
Term
Staphylococcus
Definition
heart murmurs, crippling arthritis, Gram positive coccus
Term
Treponema
Definition
in 1492 Columbus sailed the ocean blue -some blame his motley crew - for this plague in France, Spain, and of course Italy too!
Term
Legionella
Definition
in the 1976 convention, 182 contracted this disease - 29 died - you know who did it, they didn't
Term
Neisseria
Definition
Intracellular parasite, potential for infertility in male and female
Term
Chlamydia
Definition
Leading bacteria STI
Term
Chalmydia
Definition
Leading cause of PID
Term
HPV (papilloma)
Definition
Leading STI period!
Term
Clostridium
Definition
lockjaw
Term
Yersinia
Definition
may have killed half the people in London
Term
Staphylococcus
Definition
MRSA
Term
HPV (papilloma)
Definition
new vaccine targets cervical cancer
Term
Acinetobacter
Definition
normal skin flora, Gm (-) has turned into big nosocomial killer
Term
Cryptosporidium
Definition
oocysts incredibly resistant to chlorine, redesign of water filtration
Term
Neisseria
Definition
painful urination, copious puss, usually will send "the guys" to the Dr.'s
Term
Bordetella
Definition
pertussis
Term
Yersinia
Definition
pneumatic form 95% fatal
Term
Lactobacillus
Definition
protects against yeast infection
Term
Trichomonas
Definition
protozoa, STI may get it from the toilet seat
Term
Entamoeba
Definition
protozoa, water, salad bar, bloody stool > liver, lung, brain
Term
Corynebacterium
Definition
pseudomembrane, death by asphyxiation/heart failure
Term
Clostridium
Definition
puncture wounds, vaccine toxoid
Term
Legionella
Definition
respiratory disease, water the reservoir
Term
Streptococcus
Definition
rheumatic fever
Term
Influenza V
Definition
swine common mixing vessel for genetic recombination
Term
Giardia
Definition
this flagellate (mastigophora) contaminates over 90% of surface waters
Term
Chlamydia
Definition
those with gonorrhea are often co-infected with ________
Term
Rickettsia
Definition
tick - Appalachian mountain spotted fever
Term
Borrelia
Definition
up to 1/3 may not show erythyma migrans
Term
Corynebacterium
Definition
Vaccine toxoid, Gm (+) endospores
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