Term
Compound Light Microscopes |
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Definition
Light rays pass through specimen, focused by 2+ glass lenses. Maximum magnification= 1000X |
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Term
Phase Contrast Microscopes |
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Definition
A light microscope with special lenses to look at unstained, living cells. Lenses have filters that emphisizes certaion wave lengths which amplifies density variations |
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Term
Confocal Laser Scanning Microscopes |
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Definition
No need to cut the specimen. Uses a laser beam to get 2D images of "sliced" planes, producing optical "slices" of a 3D image. Uses a light microscope. |
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Term
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Definition
Uses a beam of highly energized electron to examine objects on a very fine scale.MM 300 000X. Specimin must be dead. There are two types, Transmissions and scanning ems. |
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Term
Photograph of image from EM |
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Definition
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Term
transmisson electron microscope |
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Definition
A beam of electrons is transmitted through a specimen and focused by magnetsto produce a 2D image, m= 10 000X-100 000X Specimins are sliced into sections, stained and the viewed. Tostudy the internal structures |
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Term
Scanning electron microscope |
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Definition
Sweeps/shoots a beam of electrons over an object with an electron gun to create a 3D image. Electrons bouce of specimin which is coated with a thin layer of metal, providing detail of external featcures. mm=10 000X-300 000X |
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Term
Scanning Tunnelling Microscope |
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Definition
Non-optical. Stronger magnification then an EM. Allows scientist to predict the position of individual atoms and nolecules. Also to produce a 3D image of molecules. A fine metal probe is placed near the specimen and electrons flow between the probe and atoms on the surface of the specimen. A computer interprets the info. and produces the 3D image |
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