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Microscopes
Types of microscopes
8
Biology
10th Grade
02/14/2011

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Term
Compound Light Microscopes
Definition
Light rays pass through specimen, focused by 2+ glass lenses. Maximum magnification= 1000X
Term
Phase Contrast Microscopes
Definition
A light microscope with special lenses to look at unstained, living cells. Lenses have filters that emphisizes certaion wave lengths which amplifies density variations
Term
Confocal Laser Scanning Microscopes
Definition
No need to cut the specimen. Uses a laser beam to get 2D images of "sliced" planes, producing optical "slices" of a 3D image. Uses a light microscope.
Term
Electron Microscopes
Definition
Uses a beam of highly energized electron to examine objects on a very fine scale.MM 300 000X. Specimin must be dead. There are two types, Transmissions and scanning ems.
Term
Photograph of image from EM
Definition
electron micrograph
Term
transmisson electron microscope
Definition
A beam of electrons is transmitted through a specimen and focused by magnetsto produce a 2D image, m= 10 000X-100 000X Specimins are sliced into sections, stained and the viewed. Tostudy the internal structures
Term
Scanning electron microscope
Definition
Sweeps/shoots a beam of electrons over an object with an electron gun to create a 3D image. Electrons bouce of specimin which is coated with a thin layer of metal, providing detail of external featcures. mm=10 000X-300 000X
Term
Scanning Tunnelling Microscope
Definition
Non-optical. Stronger magnification then an EM. Allows scientist to predict the position of individual atoms and nolecules. Also to produce a 3D image of molecules. A fine metal probe is placed near the specimen and electrons flow between the probe and atoms on the surface of the specimen. A computer interprets the info. and produces the 3D image
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