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mizzou bio 1500 test 2
mizzou bio 1500 test 2
250
Biology
Undergraduate 1
03/06/2012

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Term
virus (2 qualities)
Definition
not alive
strand of dan/rna in protein coat
Term
how do viruses replicate?
Definition
cannot reproduce themselves
enter cell and direct cell to make copies of the virus
Term
capsid
Definition
protein coat encasing viral genome
(additional envelope may surround capsid)
Term
capsid envelope
Definition
bilayer membrane of proteins and glycoproteins
possesses surface markers that cause them to be taken in by host cells--very specific for host cells they infect (humans get duck flu from pig...most start in china)
Term
do viruses affect all types of living organisms?
Definition
probably
Term
virulent viruses
Definition
viruses that kill cells--by directing cell to produce many copies of virus and then lyse to release viruses (LYTIC CYCLE)
Term
temperate viruses
Definition
viruses that become permanently established parts of genome without killing cells (LYSOGENIC CYCLE)
Term
how much of human genome is viral?
Definition
8%
and under certain conditions, temperate viruses may go viral
Term
why are viruses difficult to fight?
Definition
because their membranes are similar to host's--use host's system to replicate and can hide inside cells
Term
what do antiviral drugs do?
Definition
inhibit viral dna/rna replication or are protease inhibitors that block assembly of viral proteins
Term
what do rna viruses use their rna for? (indirectly or directly)
Definition
-used directly as template for mrna
Term
retroviruses and reverse transcriptase
Definition
retroviruses use reverse transcriptase to make dna copy of their rna and insert that into host's dna
Term
nucleotide
Definition
nitrogenous base
5 carbon sugar
1 phosphate group
Term
dna and rna strand make-up (4 each)
Definition
dna ATCG -- A&T and C&G -- double-stranded, long
rna AUCG -- A&U and C&G -- single-stranded, short

rna uses a ribose sugar instead of a deoxyribose sugar like dna
Term
what forms the stands and bases and cross links in dna/rna?
Definition
sugar and phosphate groups form the strands; bases form the cross links
Term
pyrimidlines
Definition
one-ring compounds
C, U, and T
Term
purines
Definition
two-ring compounds
A, G
Term
how do purines and pyrimidlines combine?
Definition
2-ring bases pair with 1-ring bases
Term
chromosomes are composed of __% dna and __% histones
Definition
40; 60
Term
nucleosome
Definition
ball-like structure of dna
Term
gene
Definition
unit of dna that tells how to build mrna, rrna, or trna or regulate transcription
Term
for protein synthesis...
each sequence of __ nucleotides codes for 1 amino acid
Definition
3
-some tell when to start/stop reading instructions
-proteins are polymers of AAs built one amino acid at a time
Term
what phase does dna replication take place in?
Definition
s-phase
Term
what is semiconservative replication?
Definition
a new double-stranded dna has 1 old and 1 new strand
Term
helicase
Definition
uses atp to untwist dna and separate double-strands

forms replication fork and bubble in dna
Term
dna polymerase
Definition
makes new strands by adding correct nucleotides to new strand and proofreading
Term
mismatch repair
Definition
dna polymerase corrects mistakes by comparing template with near strand
Term
which direction does dna polymerase synthesize a new strand?
Definition
5' - 3'
synthesizes leading strand continuously toward fork
Term
lagging strand
(discontinuous replication)
Definition
the strand that dna polymerase synthesizes in opposite direction -- then has to skip back to fork
Term
how many replication forks duplicate a dna strand at once?
Definition
100s-1000s
Term
-telomeres -what is the tag for a telomere?
Definition
non-coding nucleotides TTAGGC
Term
about __ blocks of TTAGGC (telomere tag) are lost each time a cell divides
Definition
16 (dna polymerase stops copying several hundred bases before end of chromosome)
when a cell runs out of telomeres, it dies.
telomeres are molecular clocks that encode lifespan
Term
when telomeres get short, _____ slows and cell goes into _____
Definition
mitosis; senescence
Term
telomerase
Definition
only in immortal cells: adds TTAGGCs during each mitosis and telomeres never shorten
(stem cells and cancer cells have telomerase)
Term
pair to TAGGCATCGA
Definition
ATCCGTAGCA
Term
progeria
Definition
kids age at extremely accelerated rate
most die at 13 yrs old of old-age disease lie heart and cancer
have telomeres of 80 yr old at birth
Term
transcription
Definition
production of mrna copy of a gene
(information flows from dna to rna proteins)
Term
transcription begins when ___ _________ binds to __________ __________ on DNA
Definition
RNA polymerase; promoter sequence
Term
RNA polymerase
Definition
separates DNA strands
Term
TATA box
(TATAAA)
Definition
common site of promoter location in Eukaryotes located at the edge of gene
Term
which strand is transcribed?
Definition
template strand
Term
what reads the template strand?
Definition
dna polymerase
Term
what does rna polymerase do as it moves along the template strand and reads each nucleotide?
Definition
it forms a complementary mrna chain...uses u instead of t
Term
dna is read from __ to __
rna is created from __ to __
Definition
3; 5
5; 3
Term
what causes the rna polymerase to detach at the end of a gene?
Definition
stop sequence
Term
5' cap
Definition
methylated guanine group added to 5' end of mrna
Term
what does the 5' cap do?
Definition
inhibit degradation
promote translation
is necessary for export of mrna
Term
poly A tail
Definition
adenines added to 3' end of mrna at end of transcription
Term
what is the polyA tail important for?
Definition
nuclear export
translation
stability of mrna
Term
exons
Definition
dna coding sequences (useful information)
Term
introns
Definition
dna non-coding sequences
Term
bacterial genes contain what type of coding sequences?
Definition
coding (exons)
Term
eukaryotic genes contain what type of coding sequences?
Definition
both coding and non-coding (exons and introns)
Term
rna processing
Definition
before mrna transcript leaves the nucleus, introns are cut out and the exons are spliced together
Term
translation
Definition
turns mrna nucleotide sequence into amino acid sequence
begins when mrna binds to ribosome
Term
translocation
Definition
mrna is moved through ribosome in increments of 3 nucleotides
Term
translocation moves in which direction?
Definition
5' to 3'
Term
__ nucleotides code for an amino acid that the ribosome adds to the new polypeptide chain
Definition
3
Term
to make a polypeptide of n amino acids, it takes ______ GTPs?
Definition
4n-1
Term
what brings amino acids to ribosomes?
Definition
tRNA
Term
start codon
(which codes methionine)
Definition
AUG
Term
stop codons (when polypeptide is released)
Definition
UAA
UGA
UAG
Term
codon
Definition
coding block of 3 nucleotides (in DNA or RNA)
Term
genetic code
Definition
tells which triplet codes for each amino acid
Term
how many common amino acids are used to make proteins?
Definition
20
Term
anticodons
Definition
triplets of nucleotides in trna that are complementary to mrna codons and pair with them
Term
each tRNA carries an amino acid specific to its _____________
Definition
anticodon
Term
how are polypeptides formed
Definition
tRNAs bind one after the other and add their amino acid to a chain (which becomes a polypeptide?)
Term
can tRNAs recognize more than 1 anticodon?
Definition
yes, they can.
Term
can tRNAs recognize more than 1 anticodon?
Definition
yes, they can.
Term
stop codons (their combos)
Definition
UAA UGA UAG
Term
are the first two nucleotides in a codon the same?
Definition
yes, they usually are and can "wobble" to bind to codon
Term
oldest fossil of a multicellular
what kind of living organism?
Definition
1.2 byo
algae
Term
when did the first multicellular animals probably evolve?
Definition
700mya-1bya
Term
when was the ice age (ice covered pole to pole) that supports the theory of the Snowball Earth Hypothesis?
Definition
750-500 mya
Term
what happened for multicellulars when the earth thawed?
Definition
great radiation of multicellulars (increase in number)
Term
what period did larger, soft-bodied animals appear? and about how long ago?
Definition
late Proterozoic -- 570 mya
Term
what kinds of large, soft-bodies animals appeared in the late Proterozic (570mya)?
Definition
herbivores (algae-feeders)
filter-feeders
scavengers
NO CARNIVORES
Term
when and where was there a large explosion of animal species?
Definition
500-540mya in the Cambrian Seas
Term
what did the co-evolution of predator and prey species lead to? (2 items)
Definition
evolution of exoskeletons with greater mobility
hox genes, which provided for new body plans
Term
hox genes
Definition
control body plan and appendages in animals
Term
where did the most extant (still existent) animal phyla and body plans evolved where?
Definition
Cambrian
Term
where did knowledge of the Cambrian first come from?
Definition
the Burgess Shale in Canada
Term
what its the most common burgess shale fossil?
Definition
marella (aka tritobyte)
Term
where did our chordate ancestors evolve?
Definition
Cambrian
Term
what is the most likely link to our ancestors?
Definition
Pikaia gracilens (worm)
Term
when did large plants and animals colonize land?
Definition
500 may
Term
invertebrates
Definition
animals without backbones
Term
what were the first invertebrates? (the phylum)
Definition
Ctenophora
Term
what was the first Ctenophora?
Definition
comb jellies (rows of cilia that look like combs)
Term
Ctenophora have what type of symmetry?
Definition
radial or biradial -- nerve net
Term
Porifera
Definition
sponges
no symmetry, tissues, or organs
Term
Cnidaria
Definition
jellyfish, coral builders
radial symmetry, epithelium, sac-like gut, nerve net, muscle
Term
what is the simplest organism with bilateral symmetry and organs?
Definition
Platyhelminthes (flatworms)
Term
first phyla to have cephalization and bilateral nerve cord
Definition
Platyhelminthes (flatworms)
Term
tapeworms are...?
Definition
Platyhelminthes (flatworms)
Term
first with tube digestive system?
Definition
Nematoda (roundworms)
Term
pinworms, heartworms, and Loa Loa (African eye worm) are all...?
Definition
Nematoda (roundworms)
Term
first with advanced body cavity?
Definition
mollusca (clams, squid, octopi)
Term
first with segmentation and closed circulatory system
Definition
Annelida (earthworms, leeches)
Term
greatest number of species of any animal (over 1 million)
Definition
Arthropoda (spiders, insects, crustaceans)
Term
have hard exoskeleton, joined appendages, separate feeding, and reproductive stages
Definition
Arthropoda (spiders, insects, crustaceans)
Term
which arthropoda were the most successful?
how many?
Definition
insects
800,000
Term
which phyla did humans come from?
Definition
Chordata
Term
at some time have:
nerve cord
notochord
pharynx
tail
Definition
Chordata
Term
two classes of Chordata
Definition
Tunicates and Lancelets
Term
Vertebrate
Definition
chordate with backbone and encased brain
Term
agnatha
Definition
jawless fish (lampreys)
Term
chondrichithyes
Definition
cartilaginous fish (sharks, rays)
Term
osteichthyes
Definition
bony fishes
Term
the most numerous and diverse class of vertebrate
Definition
osteichthyes
Term
amphibia
Definition
amphibians
arose from fish
lobe-finned fish first became early amphibians
Term
first to leave water
Definition
amphibians
Term
reptilia
Definition
arose from amphibians
first to not be dependent on standing water
Term
first to evolve cerebral cortex
Definition
reptiles/reptilia
Term
aves
Definition
birds
arose from dinosaurs
only vertebrates with feathers
Term
mammilia
Definition
vertebrates with hair and mammary
evolved from reptiles
Term
therapsids
Definition
ancestors of mammals
diverged from reptiles about 200 mya
Term
when did an asteroid the size of mt. everest crash into earth?
Definition
65 mya
Term
where did the mt. everest-sized asteroid crash?
Definition
yucatan peninsula
Term
what caused global broiling?
what happened as a result of global broiling and cooled earth?
Definition
an asteroid crashing into the yucatan peninsula
all land animals larger than dogs went extinct...no more dinosaurs...mammal speciation exploded
Term
3 major lineages
Definition
monotremes
marsupials
placental
Term
monotremes
Definition
egg-laying mammals
Term
marsupials
Definition
mammals with a pouch
Term
placental mammals
Definition
embryonic development is in uterus
Term
new trait seen in mammals
Definition
extended period of care and training of offspring
Term
physiology
Definition
how tissues and organs maintain homeostasis
Term
nervous system composed of 2 broad classes of cells which are...
Definition
neurons and glia
Term
neuron
Definition
functional unit of nervous system that performs information transfer
Term
glia
Definition
performs most other functions in the nervous system that the neurons don't
Term
what performs the following:
involved in regeneration of cut nerves
immune responses of CNS
insulation and nutritional support of neurons
Definition
glia
Term
what evolved to perform fast communication between the parts of animals
Definition
neurons
Term
specialized extensions of cell bodies (two types)
Definition
axons and dendrites
Term
dendrites
Definition
carry info to soma
Term
soma
Definition
neuron cell body
Term
axons
Definition
carry info from soma to other cells
Term
synapse
Definition
where dendrites and axons form contacts
where signals are received/transmitted
each neuron received 10^3 to 10^4 synapses from other neurons
Term
what does it mean to say neurons are excitable?
Definition
they can change their membrane voltage quickly to create and Action Potential
Term
how is an action potential created?
Definition
flow of Na+ into cell through v-gated na+ channels
Term
charge across plasma membrane (as result of different ion distributions on either side of membrane) during resting potential is
Definition
-55 to -100 mV
-90 mV average
Term
at resting state, where is the NaCl and K+?
Definition
NaCl outside
K+ inside
Term
ion channel
Definition
pass ions in/out of cell
some always open
some have gates that open by neurotransmitters or are voltage-gated channels
Term
ion pump
Definition
pump ions--require energy
na+/k+
ca++
Term
Vm
Definition
membrane voltage
Term
in cell, how permeable is K+?
Definition
very permeable
Term
in cell, how permeable is Na+?
Definition
not very permeable

most Na+ that leaks into cell is pumped out
Term
most protons inside the cell are too large to escape and have lots of negative charge...as a result, the cell must be balanced with lots of _________
Definition
K+ (positive ions)
Term
why doesn't the Cl- (which is permeable) move into the cell?
Definition
because it is repelled by proteins in the cell
Term
the negative resting potential results from...
Definition
a balance between concentration gradient and electrical forces acting on ions (mainly K+)
Term
at the resting potential there are still high concentration gradient and electrical forces acting on ___
Definition
Na+
Term
high concentration gradient and electrical forces acting on Na+ form driving force for the ability of excitable cells to rapidly discharge their Vm to create ______
Definition
an Action Potential
Term
what kind of cells have the innovation of voltage-gated Na+ channels?
Definition
excitable cells
Term
which gates are opened/closed at RP?
Definition
activation gate--closed
inactivation gate--open
Term
what charge is on the arms of the activation gate?
Definition
positive
Term
how does the activation gate remain closed?
Definition
positive charge on outside and negative charge inside hold the gate closed
Term
how does the activation gate open?
Definition
neuron membrane depolarizes (becomes less negative inside)
positively charged arms are repelled by positive changes inside and attracted by negative charges outside
Term
what happens when activation gate opens?
Definition
Na+ rushes in and creates an Action Potential
Term
how long does an activation gate remain open?
Definition
1 ms
Term
neuron at RP has a charge of:
at what charge is the threshold reached?
Definition
-90 mV
-50mV
Term
what happens when depolarization reaches the threshold?
Definition
an Action Potential is irreversibly fired and inactivation gate closes to stop Na+ flow
Term
what happens as the Vm repolarizes?
Definition
K+ leaves through open channels and Na+ is pumped out
Term
where do APs normally start?
Definition
the base of an axon
Term
when depolarizing, positive current flows into axon from synapses--what does this cause?
Definition
v-gated na+ channels at base of axon to open
Term
what myelinates some vertebrate axons?
Definition
glia
Term
what type of glia myelinates peripheral and central nervous systems>
Definition
peripheral--schwann cells
central--oligodendrocytes
Term
what is the difference between travel in myelinated and unmyelinated axons?
Definition
action potentials skip from node to node in myelinated axons...this speeds up conduction
Term
saltatory conduction
Definition
when APs jump from node to node in myelinated axons (faster)
Term
spaces between myelin
Definition
nodes of Ranvier
Term
the ____________________ are just bare membrane...only place that AP occurs
Definition
nodes of Ranvier
Term
Multiple Sclerosis
Definition
autoimmune disease that attacks myelin in Central Nervous System
without myelin, AP conduction is slowed or stopped
Term
synaptic knob (aka synaptic terminal)
Definition
axon terminal (aka synaptic terminal)
Term
what releases neurotransmitter?
Definition
presynaptic neuron
Term
what is neurotransmitter contained in?
Definition
vesicles
Term
when Action Potential reaches synapse, what opens?
Definition
V-gated Ca++ channels
Term
what causes exocytosis of neurotransmitter vesicles
Definition
high Ca++
Term
how do channels on the post-synaptic cleft open?
Definition
neurotransmitter binds to the channels
Term
what happens when neurotransmitters open channels for ions that depolarize?
Definition
the post-synaptic cell is excited
Term
to hyperpolarize
Definition
to make Vm more negative
Term
what happens when neurotransmitters open channels for ions that hyperpolarize?
Definition
post-synaptic cell is inhibited
ex:CL- or K+ channels
Term
classical neurotransmitters
Definition
8 small molecule transmitters
Term
neuropeptides
Definition
larger than classicals
consist of short chains of amino acids
Term
gaseous neurotransmitters
Definition
NO and CO
vasodilators; viagra and nitroglycerin affect NO levels
Term
endocannabinoids
Definition
lipids
synthesized on demand
not stored in vesicles
Term
list the 8 classical neurotransmitters
Definition
Ach
NE
dopamine
seratonin
GABA
glutamate
asparate
glycine
Term
Ach
Definition
used at all nerve/skeletal muscle synapses and in Autonomic Nervous System and brain
Term
glycine and GABA
Definition
always inhibitory
open Cl- channels
ex: sedative/tranquilizing drugs
Term
what inhibits GABA release?
Definition
caffeine
Term
glutamate and aspartate
Definition
always excitatory
Term
most common NT?
what percentage of brain synapses use it?
Definition
glutamate
90%
Term
two NTs that are chemically related
Definition
dopamine and NE (norepinephrine)
Term
dopamine is involved in what two major systems?
Definition
motor (movement)
control (by brain)
Term
dopamine is involved in what two major systems?
Definition
motor (movement)
control (by brain)
Term
dopamine is involved in what two major systems?
Definition
motor (movement) control by brain
emotional reward--linked to addiction and schitzophrenia
Term
how is schitzophrenia treated?
Definition
block one type of dopamine receptor
Term
NE (norepinephine)
Definition
involved in ANS and settling level of arousal
Term
Seratonin
Definition
emotion
mood
behavior
appetite
brain circulation (migraines)
learning
Term
persons with OCD have low levels of...?
Definition
seratonin
Term
what drug stimulates seratonin's main excitatory receptor?
Definition
LSD
Term
how many neuropeptides are there?
Definition
100+
Term
where are neuropeptides released?
Definition
sides of the synaptic knob
they don't open channels directly
Term
what do neuropeptides do?
Definition
affect specific behaviors, modulate sensory perception and emotions
Term
how do neuropeptides affect sensitivity of synapses?
Definition
control number of receptors
Term
substance P
Definition
pain neurotransmitter
Term
enkephalins
endorphin
dynorphin
Definition
opiate neurotransmitters
Term
what do opiate neurotransmitters do?
Definition
block release of substance P
mediate placebo effects
Term
analgesics
Definition
body's own painkillers
Term
what is given for drug overdose?
it is an opiate antagonist
Definition
naloxone (Narcan)
Term
neurotransmitter systems in brain
Definition
NE
dopamine
seratonin
ACh
Term
what do endocannabinoid transmitters do?
Definition
regulate activity of other NT systems involved in our experience of novelty and habituation to non-novel stimuli regulate appetite anti-nausea effects
Term
all neurons release a combo of ________ at their synapses at high rates of firing
Definition
1 classical and 1+ neuropeptide (except myelinated sensory and motor neurons)
Term
how are NTs inactivated?
Definition
enzymatic breakdown and reuptake
Term
what breaks down ACh
Definition
cholinesterase
Term
atropine
Definition
blocks one type of ACh
Term
oximes
Definition
reactivate cholinesterase
Term
what blocks cholinesterase?
Definition
some insecticides
Term
what disease is associated with loss of ACh-producing neurons in the brain?
Definition
Alzheimers
Term
how do you treat Alzheimers?
Definition
drugs that inhibit cholinesterase
Term
most common mechanism for inactivation of NTs
Definition
reuptake
Term
how does reuptake work?
Definition
synaptic knob that releases NT also has pumps that retrieve it
Term
how do addictive substances work?
Definition
inhibit dopamine reuptake transporter
Term
are neuropeptides inactivated slowly or quickly?
Definition
slowly
Term
what is depression accompanied by?
Definition
imbalance of NTs
Term
what main NTs are involved in depression?
Definition
dopamine, seratonin, NE
Term
what do antidepressants do?
Definition
block reuptake of NE (and some block seratonin reuptake)
Term
what do cocaine and amphetamines do?
Definition
inhibit reuptake of seratonin, dopamine, NE
Term
SSRIs
Definition
selective seratonin reuptake inhibitors
increase seratonin levels
Term
how many seratonin subtype receptors are there?
Definition
12
Term
SSRIs
NDRIs
SNRIs
Definition
seratonin reuptake inhibitor
NE/dopamine reuptake inhibitor
seratonin/NE reuptake inhibitor
Term
sensitization
Definition
a NT antagonist causes increase in number and sensitivity of receptors for that NT
Term
desensitization/tolerance
Definition
antagonists cause number and sensitivity of receptors to decrease
Term
muscle fiber
Definition
muscle cell
multinucleate
Term
how many myofibrils per muscle fiber?
Definition
about 100
Term
these are full of actin and myosin
Definition
myofibrils
Term
why are skeletal muscles striated?
Definition
because myofibrils are precisely aligned
Term
muscle fibers are large and multinucleate because they
Definition
are formed by fusion of many muscle cells during development--adaptation for generating power
Term
is skeletal muscle excitable?
Definition
yes
Term
how often is threshold reached when a motor neuron fires?
Definition
always
Term
muscle cell RPs and thresholds are like....
Definition
axons
Term
thinner of the two filaments in myofibrils
Definition
actin
Term
z-line
Definition
structural protein that anchors actin
Term
sarcomere
Definition
functional unit of skeletal muscle
from one z-line to the next
Term
crossbridge cycle
Definition
bind...
ratchet...
unbind...
...of myosin to actin
Term
at rest, what is the position of the myosin head?
Definition
cocked
Term
what breaks the cross bridge and rechecks myosin?
Definition
ATP
Term
what separates myosin from actin?
what causes structural change that rids this separator?
Definition
tropomyosin
4 Ca++s added to troponin
Term
at what molarity does the cross bridge cycle begin and continue?

when does relaxation occur?
Definition
[Ca++] > 10^-7M

10^-7M
Term
where is Ca++ released from?
Definition
sarcoplasmic reticulum
Term
T-tubules Action Potential causes...
Definition
SR to release Ca++
Term
mechanical link in T-tubule system...
Definition
opens Ca++ channels in adjacent SRs
Term
smooth muscle vs. skeletal muscle
Definition
smooth:
no striations
much thinner
single nucleus
involuntary
spontaneously active--no troponin
ANS modulates spontaneous activity
good for slow(10x slower), sustained contractions using less energy
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