Term
Define the following:
Exonuclease |
|
Definition
| Enzyme that digests nucleic acids starting at the 5' or 3' end and extending inward |
|
|
Term
Define the following:
Endonuclease |
|
Definition
| Enzyme that digests nucleic acids at specific sequences within the DNA |
|
|
Term
| What are Restriction endonucleases (Restriction enzymes)(RE) produced by? |
|
Definition
|
|
Term
| What do Restriction endonucleases (Restriction enzymes)(RE) protect against? |
|
Definition
| Infection by bacteriophages (bacterial viruses) |
|
|
Term
| What do Restriction endonucleases (Restriction enzymes)(RE) restrict? |
|
Definition
|
|
Term
True or False:
Restriction endonucleases work on ALL DNA regardless of source |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Cuts DNA at specific sequences |
|
|
Term
|
Definition
|
|
Term
| Which RE Type is the most useful for molecular techniques? |
|
Definition
|
|
Term
|
Definition
| Enzyme digests (cuts) DNA at a specific sequence |
|
|
Term
| RE Palindromic Restriction Sites |
|
Definition
| Double-stranded DNA palindromic sequence reads the same 5' to 3' on both strands |
|
|
Term
| Double-stranded DNA palindromic sequence reads the same 5' to 3' on ____ strands |
|
Definition
|
|
Term
| RE recognizes 4 to ____ base pairs, palindromic sequences |
|
Definition
|
|
Term
| What is the Name of REs based on? |
|
Definition
| Bacterial genus, species, strain, and enzyme number |
|
|
Term
Given the following determine the name:
Genus: Escherichia
Species: coli
Strain: RY13
Enzyme #: 1st RE isolated from strain |
|
Definition
|
|
Term
| Which of the enzymes covered in lecture are considered blunt end cutters? |
|
Definition
|
|
Term
| What is the recognition sequence for HaeIII? |
|
Definition
|
|
Term
| What is the recognition sequence for PvuII? |
|
Definition
|
|
Term
| What is the recognition sequence for EcoRI? |
|
Definition
|
|
Term
| What is the recognition sequence for PstI? |
|
Definition
|
|
Term
| Which of the enzymes covered in lecture are considered to make staggered cuts / "Sticky ends"? |
|
Definition
|
|
Term
True or False:
Average distance between cuts is 4^n, where "n" is the number of base pairs in the recognition site |
|
Definition
|
|
Term
| Frequency of Enzyme Restriction Sites within Genomic DNA for a 4-base cutter would be? |
|
Definition
|
|
Term
| Frequency of Enzyme Restriction Sites within Genomic DNA for a 5-base cutter would be? |
|
Definition
|
|
Term
| Frequency of Enzyme Restriction Sites within Genomic DNA for a 6-base cutter would be? |
|
Definition
|
|
Term
| The _____ the recognition site, the more often the base pair recognition sequence occurs within a DNA sequence. |
|
Definition
|
|
Term
True or False:
Generated fragments from RE can be rejoined |
|
Definition
|
|
Term
True or False:
RE is the basis of recombinant DNA technology and genetic engineering |
|
Definition
|
|
Term
List the following steps of preparing recombinant DNA into the correct order:
Vector such as a plasmid isolated.
Bacterial cells with gene of interest are cloned.
Gene is inserted into plasmid to form Recombinant DNA; ligase used to connect gene to plasmid.
DNA containing gene of interest is cleaved by an enzyme into fragments.
Plasmid taken up by a cell such as a bacterium. |
|
Definition
Vector such as a plasmid isolated.
DNA containing gene of interest is cleaved by an enzyme into fragments.
Gene is inserted into plasmid to form Recombinant DNA; ligase used to connect gene to plasmid.
Plasmid taken up by a cell such as a bacterium.
Bacterial cells with gene of interest are cloned. |
|
|
Term
| What are Clones used for? |
|
Definition
*Making copies of gene, which are harvested & used for a variety of reasons
*Making gene's protein product, which is harvested & used for variety of reasons
*DNA libraries |
|
|
Term
| List some of the reasons why a copy of the gene is used |
|
Definition
Probes for hybridization techniques
Insert gene into plant for pest resistance |
|
|
Term
| List some of the reasons why a copy of the gene's protein product is used |
|
Definition
Human insulin
Human growth factor
Blood clotting factor VIII
tPA (Clot-busting)
Interferon
Taxol
Hepatitis A & B vaccines |
|
|
Term
| Explain the DNA libraries |
|
Definition
(a) Collection of clones
(b) Each clone has a different gene
(c) Goal to have every gene in an organism in a clone
(d) Can map & study an organism's genes |
|
|
Term
Define the following:
Restriction Fragment Length Polymorphisms (RFLPs) |
|
Definition
| DNA fragments produced by RE |
|
|
Term
Define each section of RFLPs:
Restriction |
|
Definition
|
|
Term
Define each section of RFLPs:
Fragments |
|
Definition
| DNA cut into pieces by RE |
|
|
Term
Define each section of RFLPs:
Length |
|
Definition
| DNA pieces vary in length |
|
|
Term
Define each section of RFLPs:
Polymorphism |
|
Definition
| RFLPs polymorphic in that different individuals have RFLPs of different lengths |
|
|
Term
| Are RFLPs Polymorphism inherited or acquired? |
|
Definition
|
|
Term
|
Definition
DNA Profiling
Diagnosis of a few genetic diseases
Microorganism fingerprinting |
|
|
