Term
| Method to introduce foreign genes into plants |
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Definition
Using Ti Plasmid to produce Transgenic Plants:
1) Ti Plasmid isolated from Agrobacterium tumefaciens . T DNA (segment of DNA that integrates into host cell)
2) The foreign gene of interest is inserted into the middle of the T DNA using restriction enzymes
3) The recombinant Ti Plasmid can be introduced into the cultured plant cells by electroporation or by returning it into Agrobacterium and infecting the plant |
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Term
| Agrobacterium Infection Summary |
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Definition
Agrobacterium enters at wound site
Agrobacterium binds to plant cell wall
T-DNA copied from Ti plasmid
T-DNA enters plant cell
T-DNA integrates into chromosomal DNA |
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Term
ENVIRONMENTAL REGULATION
OF GENE EXPRESSION |
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Definition
1) CAB Gene is induced by Light
2) Stress Induced Gene
3) Touch Induced Gene
4) |
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Term
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Definition
1) Small and easy to grow
2) rapid generation time ( 6 weeks)
3) hundreds of seeds per plant
4) self fertile and also can be crossed
5) easy to produce mutants
6) |
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Term
| Why is Arabidopsis so good for genetic approaches? |
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Definition
1) Small Genome (little repetitive DNA)
2) easily genetically transformed using Agrobacterium
3) Genes corresponding to mutants can be isolated
4) |
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Term
| Importance of light in gene expression/development |
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Definition
1) 1/3 of genome are regulated by light
e.g. CAB protein, chlorophyll
2) |
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Term
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Definition
Light presence/absence
Light quantity
Light spectral quality
Light direction
Light duration |
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Term
| What does Phytochrome regulate? |
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Definition
Phytochrome regulates CAB
gene transcription . Phytochrome is involved in controlling
flowering time |
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Term
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Definition
Cryptochromes bind flavin and pterin
chromophores that absorb UV-A and blue
light.
involved in controlling
processes such as stem extension, gene
expression and flowering time |
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Term
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Definition
Phototropins bind flavin chromophores
that absorb mainly UV-A and blue light.Also controls plant responses: phototropism, stomatal opening, and chloroplast movement |
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Term
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Definition
| Plants use biological clocks to regulate processes during the day and detect seasonal changes to regulate flowering developmental changes (e.g.flowering) |
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