Term
Pulse labeling experiments Dintzis |
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Definition
demonstrated that polypeptides grew from their N-termini to their C-termini amino acids incorporated in a brief labeling period were shown to be detectable in peptides derived from the C-terminus of full lenth B-globin before they appeared in N-terminal peptides. [image] |
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Term
| what experiment to determine directionality of translation? |
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Definition
| Established by harboiring a unique 3' nucleotide ([Ap]nC-->N-(lys)n-asn-C, not the opposite[image] |
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Term
| name the components of the prokaryotic and eukaryotic translational apparatus |
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Definition
prokaryotes: 70s ribosomes with 30s and 50s subunits >50 ribosomal proteins Eukatyotes: 80s ribosomes with 40s and 60s subunits >70 ribosomal proteins |
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Term
| How do you know if the strucure or the sequence of the RNA is the cause of the reaction (i.e.-rRNA)? |
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Definition
first way: use RNase T1 (which cleaves single stranded RNA) to see if there are loops and/or ds binding second way: can mutate and see the behavior of the rxn then do a rescue to see if the helix is critical third way: can look at the sequencing of other sequences |
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Term
What is the structure of the tRNA? |
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Definition
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Term
| which codon permits wobble pairing and which don't? |
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Definition
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Term
Aminoacyl-tRNA synthetases (how to tRNA get amino acids attached?) |
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Definition
soluble enzymes that catalyze a two-step esterification of amino acids to tRNAs. In general, there is one synthetase per amino acid (insert figure here) |
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