Term
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Definition
- sperm acquire fertility in female tract
- acrosome alteration
- not fully reversible
- 50-240 minutes
- initiated in cervix by seminal plasma
- completed in isthmus during detachment from sperm reservoir by female derived factors |
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Term
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Definition
-increase in flagellar bend amplitude & asymmetry
-enhances release from sperm reservoir, progressive motility & sperm penetration of cumulus ECM
-results in tryosin phosphorylation of AKAP83 & other proteins
-reversible |
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Term
| oocyte transport controls |
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Definition
-estrogen domination
-isthmus & uterus have lots of sympathetic nerves
-stimulatory (alpha) & inhibitory (beta) receptors increase
-stimulatory --> contraction of isthmus
-inhibitory --> relaxation of isthmus --> embryo transported to uterus |
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Term
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Definition
-chemo-attractants may be present in follicular fluid that accompanies the eggs
-sperm get chemotactic responsiveness |
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Term
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Definition
secondary difference between isthmus & ampulla
exposure to cumulus cells alter sperm motility |
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Term
| 4 phases of oocyte maturation |
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Definition
1. mitotic divisions of primordial germ cells
2. nuclear arrest
3. cytoplasmic growth
4. resumption of meiosis following LH surge |
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Term
| primoridal germ cells (2n) |
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Definition
-migrate to developing gonads
-10-20 um
-mitotic divisions (start with a few, end with millions)
-after mitotic stage (dna snythesis) |
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Term
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Definition
-progress to 1st mitotic prophase
-arrest in prophase 1 (pause button) |
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Term
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Definition
-primary oocyte
-single layer squamous epithelial cells
-primordial resting pool |
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Term
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Definition
-primary oocyte
-single layer of 11-20 cuboidal granulosa cells
-begin to increase in organelles (smooth, rough er, elongated & dividing mitochondria) |
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Term
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Definition
-primary oocyte
-2nd layer of granulosa cells
-initial deposition of zona pellucida glycoproteins
-oocyte begins RNA synthesis |
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Term
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Definition
made of glycoproteins PRODUCED BY THE OOCYTE
-sperm bind to zona protein 3 |
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Term
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Definition
primary oocyte
oocyte begins to grow, becomes transcriptionally active |
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Term
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Definition
primary oocyte
oocyte growth stops
perivitelline space appears
free smooth and rough er decrease
hooded mitochondria appear
mitochondria, golgi bodies, cortical granule clusters & migrate to periphery |
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Term
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Definition
primary oocyte
increase in lipid content
decrease in size of golgi
cortical granules move closer to plasma membrane |
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Term
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Definition
cortical granules move inside of plasma membrane
perivitelline spaces completely formed
increase in lipid content
decrease in golgi bodies
*meiosis resumed |
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