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Section 2: Part 4
Additional Questions & Charts
55
Biology
Undergraduate 4
08/25/2015

Additional Biology Flashcards

 


 

Cards

Term
With respect to detecting vancomycin resistance in enterococci:
What is the recommended incubation time?
Definition
24 Hours
Term
With respect to detecting vancomycin resistance in enterococci:
Should transmitted or reflected light be used to examine the zone of inhibition in the agar disk diffusion test?
Definition
Transmitted Light
Term
With respect to testing Enterobacteriaceae:
How are extended-spectrum beta-lactamases (ESBLs) detected using disk diffusion test?
Definition
> or = to 5 mm increase in zone diameter for either agent tested in combination with Clavulanic acid vs. its zone when tested alone. If present, all penicillins, cephems, and aztreonam are reported as resistant
Term
With respect to testing Enterobacteriaceae:
What is the purpose of the Modified Hodge Test?
Definition
Confirms carbapenemase-producing MOs. Enzyme inactivates carbapenem drugs - if present, all beta-lactamases are reported as resistant
Term
For each of teh following organisms indicate whether it is typically susceptible or resistant to the listed antimicrobial agent:
Enterobacter to Ampicillin
Definition
Resistant
Term
For each of teh following organisms indicate whether it is typically susceptible or resistant to the listed antimicrobial agent:
Enterobacter to First-generation Cephalosporins
Definition
Resistant
Term
For each of teh following organisms indicate whether it is typically susceptible or resistant to the listed antimicrobial agent:
Klebsiella to Ampicillin
Definition
Resistant
Term
For each of teh following organisms indicate whether it is typically susceptible or resistant to the listed antimicrobial agent:
Klebsiella to Carbenicillin/ticarcillin
Definition
Resistant
Term
For each of teh following organisms indicate whether it is typically susceptible or resistant to the listed antimicrobial agent:
Stenotrophomonas maltophilia to Trimethoprim-sulfamethoxazole
Definition
Susceptible
(Frequently Resistant to other drugs though)
Term
For each organism or group of organisms listed, what is the appropriate medium?
Enterics & Pseudomonas aeruginosa
Definition
Mueller-Hinton (MH) Broth (cation supplemented) or Agar
Term
For each organism or group of organisms listed, what is the appropriate incubation atmosphere?
Enterics & Pseudomonas aeruginosa
Definition
Ambient Air
Term
For each organism or group of organisms listed, what is the appropriate medium?
Staphylococci
Definition
MH Broth (cation supplemented) or Agar
(4% NaCl in Oxacilling Salt Agar Screen)
(2% NaCl in broth for Oxacillin Resistance)
Term
For each organism or group of organisms listed, what is the appropriate incubation atmosphere?
Staphylococci
Definition
Ambient Air
Term
For each organism or group of organisms listed, what is the appropriate medium?
Enterococci
Definition
MH Broth (cation supplemented) or Agar
Term
For each organism or group of organisms listed, what is the appropriate incubation atmosphere?
Enterococci
Definition
Ambient Air
Term
For each organism or group of organisms listed, what is the appropriate medium?
Streptococcus pneumoniae
Definition
MH Blood
Term
For each organism or group of organisms listed, what is the appropriate incubation atmosphere?
Streptococcus pneumoniae
Definition
5% CO2
Term
For each organism or group of organisms listed, what is the appropriate medium?
Haemophilus
Definition
HTM Agar
Term
For each organism or group of organisms listed, what is the appropriate incubation atmosphere?
Haemophilus
Definition
5% CO2
Term
What is the oxidase reaction for the Enterobacteriaceae?
Definition
Oxidase =
Term
What is the OF Glucose Reaction for the Enterobacteriaceae?
Definition
Oxidase Fermenter
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Ampicillin
Definition
Beta-Lactam
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Amikacin
Definition
Aminoglycoside
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Cefazolin
Definition
Beta-Lactam
Cephalosporin
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Gentamicin
Definition
Aminoglycoside
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Imipenem
Definition
Beta-Lactam
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Methicillin
Definition
Beta-Lactam
Penicillinase-resistant penicillin for staphylococci
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Oxiacillin
Definition
Beta-Lactam
Penicillinase-resistant penicillin for staphylococci
Term
(A drug may belong to more than one category)
Match each antimicrobial agent with its drug category:
Tobramycin
Definition
Aminoglycoside
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
Haemophilus influenzae - ampicillin
Definition
Appropriate
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
E. coli - ampicillin
Definition
Inappropriate
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
Neisseria gonorrhoeae - penicillin
Definition
Appropriate
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
Pseudomonas aeruginosa - carbenicillin
Definition
Inappropriate
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
S. aureus - penicillin
Definition
Appropriate
Term
For each of the following organism-drug pairs, indicate whether or not a Beta-Lactamase test can be used to detect that organisms's resistance to that particular antimicrobial agent:
Moraxella catarrhalis - ampicillin
Definition
Appropriate
Term
What does AST stand for?
Definition
Antimicrobial Susceptibility Tests
Term
What types of specimens are appropriate for anaerobic culture?
Definition
Specimens free from contamination.
Placed in anaerobic transport media.
Term
Give four examples of specimens that are appropriate for anaerobic cultures.
Definition
Normally sterile body fluids.
Aspirates.
Transtrachaeal aspirates.
Suprapubic aspirates.
Tissue and Biopymaterial.
Blood.
Term
What type of specimens are inappropriate for anaerobic culture?
Definition
Specimens that are contaminated with normal flora.
Term
Give four examples of specimens that are inappropriate for anaerobic cultures.
Definition
Sputum. Clean catch or catheter urine. Stool or rectal swabs. Vaginal, cervical, or urethral. Bronchial washings. Throat or nasopharyngeal swabs. Material from superficial skin sites.
Term
What oxygen indicator is included in anaerobic transport media?
Definition
Resazurin
Term
What color is this oxygen indicator that is included in anaerobic transport media when it is exposed to oxygen?
Definition
Pink in presence of Oxygen
Term
With respect to antibiotic-associated diarrhea and pseudomembranous colitis, Which organism is associated with this disease?
Definition
Clostridium difficile
Term
With respect to antibiotic-associated diarrhea and pseudomembranous colitis, What is the pathogenic mechanism (e.g., invasion, toxic production, etc.)
Definition
Produces toxins
Term
What is the oxygen indicator strip that is included in anaerobe jars and chambers?
Definition
Methylene Blue
Term
What color does the oxygen indicator strip that is included in anaerobe jars and chambers turn under aerobic conditions?
Definition
Blue
Term
What color does the oxygen indicator strip that is included in anaerobe jars and chambers turn under anaerobic conditions?
Definition
White
Term
What catalyst is used to generate an anaerobic atmosphere in anaerobe jars?
Definition
Palladium
Term
Can Palladium be used repeatedly?
Definition
Yes
Term
If Palladium can be used repeatedly, under what conditions?
Definition
If it is regenerated by heating in oven @ 160 degrees Celsius for 2 hours
Term
How long should anaerobe jars be incubated before being opened?
Definition
48 hours
Term
True or False:
Nichrome loops and needles are recommended when subculturing anaerobic bacteria
Definition
False
Term
Looking at the diagram of the aerotolerance test, featured below, what organism is an anaerobe?
[image]
Definition
Organism B is an anaerobe
Term
Which Beta-lactamase test is considered to be the best?
Definition
Nitrocefin (cefinase)
Term
What color does Nirtocefin turn when it hass a positive result?
Definition
Red
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