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synthesis of proteins; centriole replication |
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synthesis of machinery necessary for cell division; complete centriole "maturation |
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semiconservative replication |
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each strand of existing DNA serves as a template for replication of a new strand of DNA. At the end of replication process,two double helix DNAs have been formed from the original DNA |
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unwind and separate the two strands of DNA |
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Replication of dna begins with what? |
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formation of RNA primer performed by a primase enzyme and then the DNA polymerase can mind to RNA primer |
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Which strand does DNA polymerase synthesize? |
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Leading strange, continuous and smooth because DNA molecule has a specific polarity or oreination |
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What is the function of the ligase enzyme? |
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ties together the fragments of the lagging strand called Okazaki fragments to complete continuity of the replicated DNA |
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Finally the histone proteins reconfigure around the newly duplicated DNA |
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duplicated halves of the DNA are now referred to as sister chromatids they are joined in the center by centromere to form final chromosome |
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organization of mitotic spindles centrioles separate; extend microtubules, nuclear membrane breaks up |
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where do microtubules attach to? |
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part of centromere called kinetochore |
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unique sequence of DNA bound to protein |
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chromosomes aligned in center, microtubules extend from kinetochores of centromers of chomosome to centrioles. each side |
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separation of sister chromatids "pulled" by microtubules |
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Definition
uncoild chromosome, reform nuclear membrane around daughter chromosomes |
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Cytokinesis, what causes cleavage furrow and squeezes cell apart |
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Definition
cell membrane division to two daughter cells actin |
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name control mechanisms which limit cell replication |
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surface to volume ratio- critical cell size, inadequate meeting of nutritional needs contact inhibition- stops proliferation, when cells touch e/o stops multiplying/rapidly process regulatory proteins- proteins whose concentrations and or activity increase/decrease thorughout cell cycle to effect preparation and execution of cell division cyclins cdk-cylcin dependent kinases |
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Does cyclin increase, decrease, both, or stay constant during cell cycle? |
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increase and decrease concentration of cyclin |
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Are cdks constant or vary during cell cycle? |
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DNA serves as the blueprint for protein synthesis |
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a segment of DNA which serves as the blueprint for a single polypeptide
blueprint for each amino acid is 1 triplet of DNA as shown |
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synthesisze mRNA from DNA template in order to transfer info from DNA to RNA. Occurs in the nucleus |
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synthesize a protein off of mRNA template. Occurs in cytoplasm. Uses mRNA, tRNA, and ribosome |
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Which base is exposed during transcription? |
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Definition
nitrogen
DNA molecule is unzipped and nitrogen bases are exposed RNA polymerase- synthesizes mRNA; complimentary base pairing; G:C, A:U |
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What is the role of RNA polymerase in transcription |
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mRNA is a mirror image of DNA |
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three sequential bases reprsent this, which will ultimately code for an amino acid. many codons are found on one mRNA strand |
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The synthesized product of transcription is actually a pre-mRNA which is edited or shortened to form the mRNA which will be used for transcription |
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non-informational regions;edited out |
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informational regions; left in and spliced together |
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ribosomes bind with mRNA to begin process of translation. Ribosomes contain two subunits large and small |
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Which is the first binding site for tRNA |
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What is the 2nd binding sight for tRNA |
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3rd binding site for tRNA? |
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What does the tRNA carry? |
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anticodons and amino acid each anticodon is specific for each amino acid |
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multiple ribosomes can bind with mRNA; allows formation of multiple copies of a specific protein from one template |
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Definition
fluid and matrix which make up connective tissue |
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developmental aspect of cells |
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cell differentiation hyperplasia - number of cells increase hypertrophy- size of cell increase atrophy- los of size/no longer functional cell aging and apoptosis |
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